Six water-insoluble (1→3)-β-D-glucan fractions TM8-1 to TM8-6 with weight-average molecular mass Mw ranging from 5.76 to 77.4×104 obtained from the sclerotia of Pleurotus tuber-regium were sulfated to produce the water-soluble fractions S-TM8-1 to S-TM8-6 with Mw from 6.0 to 64.8×104. The degree of substitution (DS) of S-TM8 fractions was analyzed by elemental analysis (EA) to be 1.14–1.74. The 13C NMR results indicated that the C-6 was fully substituted, and C-2, C-4 were partially substituted by the sulfo-groups. The Mw and the intrinsic viscosity [η] of the S-TM8 fractions were measured, respectively, by size-exclusion chromatography combined with laser light scattering (SEC-LLS), LLS and viscometry in phosphate buffer solution (PBS) at 37 °C. The dependences of [η] and radius of gyration 〈s2〉z1/2 on Mw for the S-TM8 samples were found to be [η]=1.89×10−2Mw0.70 (cm3/g) and 〈s2〉z1/2=1.12×10−4Mw0.81 (nm) in the Mw range tested. Based on current theories for a wormlike chain model, the molar mass per unit contour length ML and persistence length q of the S-TM8 were calculated to be 990 nm−1 and 8.5 nm, respectively. The relatively higher q value suggested a more expanded flexible chain of S-TM8 in PBS. The water-solubility and relatively expanded chain conformation of the STM8 fractions were considered to be significant to their antiviral activity. Water-insoluble (1→3)-β-D-glucans having different Mw values from the sclerotia of P. tuber-regium were sulfated to afford water-soluble derivatives. The modified β-glucans were shown by SEC-LLS and viscometry to have a more expanded flexible chain in aqueous solution than the native polysaccharides.

conformation, molecular mass, β-D-glucan, sulfonation, Pleurotus tuber-regium

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
	bDGlcp	103.0	72.5	86.3	68.5	76.1	60.9


1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

[*]O[C@@H]1[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]1O

162.141 g/mol (C₆H₁₀R₂O₅, R = next and previous repeats, not counted in mol weight)

Six water-insoluble (1→3)-β-D-glucan fractions TM8-1 to TM8-6 with weight-average molecular mass Mw ranging from 5.76 to 77.4×104 obtained from the sclerotia of Pleurotus tuber-regium were sulfated to produce the water-soluble fractions S-TM8-1 to S-TM8-6 with Mw from 6.0 to 64.8×104. The degree of substitution (DS) of S-TM8 fractions was analyzed by elemental analysis (EA) to be 1.14–1.74. The 13C NMR results indicated that the C-6 was fully substituted, and C-2, C-4 were partially substituted by the sulfo-groups. The Mw and the intrinsic viscosity [η] of the S-TM8 fractions were measured, respectively, by size-exclusion chromatography combined with laser light scattering (SEC-LLS), LLS and viscometry in phosphate buffer solution (PBS) at 37 °C. The dependences of [η] and radius of gyration 〈s2〉z1/2 on Mw for the S-TM8 samples were found to be [η]=1.89×10−2Mw0.70 (cm3/g) and 〈s2〉z1/2=1.12×10−4Mw0.81 (nm) in the Mw range tested. Based on current theories for a wormlike chain model, the molar mass per unit contour length ML and persistence length q of the S-TM8 were calculated to be 990 nm−1 and 8.5 nm, respectively. The relatively higher q value suggested a more expanded flexible chain of S-TM8 in PBS. The water-solubility and relatively expanded chain conformation of the STM8 fractions were considered to be significant to their antiviral activity. Water-insoluble (1→3)-β-D-glucans having different Mw values from the sclerotia of P. tuber-regium were sulfated to afford water-soluble derivatives. The modified β-glucans were shown by SEC-LLS and viscometry to have a more expanded flexible chain in aqueous solution than the native polysaccharides.

conformation, molecular mass, β-D-glucan, sulfonation, Pleurotus tuber-regium

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

[*]O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]3O)O[C@H](CO[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@@H]1O

648.564 g/mol (C₂₄H₄₀R₂O₂₀, R = next and previous repeats, not counted in mol weight)