Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: extracellular fluid
The structure was elucidated in this paperPublication DOI: 10.1016/S0008-6215(03)00240-4Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: corradi
prudente.unesp.br
Institutions: Departamento de Bioquı́mica, Universidade Estadual de Londrina, CCE, CEP 86051-990 Londrina, PR, Brazil, Departamento de Bioquı́mica, Universidade do Oeste Paulista, CEP 19050-900 Presidente Prudente, SP, Brazil, Departamento de Fı́sica, Quı́mica e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CEP 19060-900 Presidente Prudente, SP, Brazil
The exopolysaccharide, Botryosphaeran, produced by the ligninolytic, ascomycetcous fungus Botryosphaeria sp., was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and C-13 NMR spectroscopy showed that all the glucosidic linkages were in the beta-configuration. Data from methylation analysis and Smith degradation indicated that Botryosphaeran was a (1 --> 3)-beta-(D)-glucan with approx 22% side branching at C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consisted of single (1 --> 6)-beta-linked glucosyl, and (1 --> 6)-beta-linked gentiobiosyl residues.
exopolysaccharide, (1 -> 3;1 -> 6)-beta-glucan, Botryosphaeran, Botyrosphaeria sp., Ascomyceteous fungus
Structure type: homopolymer
Location inside paper: abstract, p.1694
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, callose, laminarin, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, cell wall glucan, polysaccharide, glycoside, β-glucan, β-1, 3-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, methylation, partial acid hydrolysis, GC-MS, sugar analysis, Smith degradation, FTIR, GPC, enzymatic digestion, HPAEC-PAD, acetylation
Comments, role: Polysaccharide obtained after Smith degradation of botryosphaeran.
Related record ID(s): 41369, 41385
NCBI Taxonomy refs (TaxIDs): 45132Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 303(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.0 86.3 68.7 76.7 61.1
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.0 | 86.3 | 68.7 | 76.7 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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