Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 38583831Publication DOI: 10.1016/j.ijbiomac.2024.131419Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Prashanth KVH <harish
cftri.res.in>, Negi PS <psnegi
cftri.res.in>
Institutions: Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India, Department of Fruit and Vegetable Technology, CSIR-Central Food Technological Research Institute, Mysuru, India, Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysuru, India
The booming mushroom industry envisages economic merits, and massive unutilized waste production (~ 20 %) creates an opportunity for valorization. Chitosan, a bioactive polysaccharide, has drawn immense attention for its invaluable therapeutic potential. Thus, the present study was conducted to extract chitosan from mushroom waste (MCH) for its prebiotic potential. The structural characterization of MCH was carried out using NMR, FTIR, and XRD. The CP/MAS-13CNMR spectrum of MCH appeared at δ 57.67 (C2), 61.19 (C6), 75.39 (C3/C5), 83.53 (C4), 105.13 (C1), 23.69 (CH3), and 174.19 (C = O) ppm. The FTIR showed characteristic peaks at 3361 cm-1, 1582 cm-1, and 1262 cm-1 attributed to -NH stretching, amide II, and amide III bands of MCH. XRD interpretation of MCH exhibited a single strong reflection at 2θ = 20.19, which may correspond to the "form-II" polymorph. The extracted MCH (~ 47 kDa) exhibited varying degrees of deacetylation from 79 to 84 %. The prebiotic activity score of 0.73 to 0.82 was observed for MCH (1 %) when supplemented with probiotic strains (Lactobacillus casei, L. helveticus, L. plantarum, and L. rhamnosus). MCH enhanced the growth of Lactobacillus strains and SCFA's levels, particularly in L. rhamnosus. The MCH also inhibited the growth of pathogenic strains (MIC of 0.125 and 0.25 mg/mL against E. coli and S. aureus, respectively) and enhanced the adhesion efficiency of probiotics (3 to 8 % at 1 % MCH supplementation). L. rhamnosus efficiency was higher against pathogens in the presence of MCH, as indicated by anti-adhesion assays. These findings suggested that extracted polysaccharides from mushroom waste can be used as a prebiotic for ameliorating intestinal dysbiosis
Lactic acid bacteria, mushroom, prebiotic, сhitosan, short chain fatty acid, waste valorization
Structure type: homopolymer
Location inside paper: p.5, p.6
Trivial name: chitosan
Compound class: O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_137340,IEDB_141807,IEDB_151531
Methods: 13C NMR, deacetylation, GC-MS, X-ray, acid hydrolysis, biological assays, HPLC, viscosity measurement, extraction, elemental analysis, spectrophotometry, SEM, centrifugation, TGA, DSC, adherence assay, FT-IR, Lowry method, titration, deproteinization, citotoxicity assay
Biological activity: compound exhibited potential prebiotic activity in terms of its utilization by various LAB strains
Related record ID(s): 44877, 44886, 46311, 46570, 46683, 48760, 48774, 49133, 49502, 49512, 49524, 49653, 50016, 50320
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G82804CF
Show glycosyltransferases
[as TSV]
13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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