Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 38677677Publication DOI: 10.1016/j.ijbiomac.2024.131799Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Yin JY <yinjy
ncu.edu.cn>; Nie SP <spnie
ncu.edu.cn>
Institutions: State Key Laboratory of Food Science and Resources, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, Nanchang, China, Food Laboratory of Zhongyuan, Henan, China
Polysaccharides are the important bioactive macromolecules in Agrocybe cylindracea, but their changes are as yet elusive during developmental process. This study investigated the dynamic changes of polysaccharides from A. cylindracea fruiting body water extract at four developmental stages and its structure characteristics. Results revealed that the polysaccharides from A. cylindracea water extract significantly increased at the pileus expansion stage and the increased fraction could be α-glucan. The further purification and identification indicated that this α-glucan was a glycogen. It had typical morphology of β particles with a molecular weight of 1375 kDa. Its backbone comprised α-D-(1→4)-Glcp and α-D-(1→4,6)-Glcp residues at a ratio of 5:1, terminated by α-D-Glcp residue. Rheological behavior suggested that it was a Newtonian fluid at the concentration of 1 %. In addition, despite both the glycogen and natural starch were composed of D-glucose, they exhibited the entirely distinct Maltese cross characteristic and unique crystalline structure. This study is the first to demonstrate the presence of abundant glycogen in the pileus expansion stage of A. cylindracea, which provides new insights on the change patterns of fungal polysaccharides
Agrocybe cylindracea, α-glucan, developmental stage, structure change
Structure type: structural motif or average structure ; 1375000
Location inside paper: Fig. 5, Table 4
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420419,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, X-ray, acid hydrolysis, enzymatic digestion, viscosity measurement, extraction, optical rotation measurement, acetylation, methylation analysis, reduction, HPGPC, precipitation, phenol-sulfuric acid assay, carbazole-sulfuric acid assay, SEM, TEM, centrifugation, ion chromatography, FT-IR, iodine staining, polarized light microscopy
NCBI Taxonomy refs (TaxIDs): 1593264
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
4,4,4,4,4 aDGlcp 99.70 71.60 73.28 76.65 71.22 60.38
4,4,4,4 aDGlcp 99.70 71.60 73.28 76.65 71.22 60.38
4,4,4 aDGlcp 99.70 71.60 73.28 76.65 71.22 60.38
4,4 aDGlcp 99.70 71.60 73.28 76.65 71.22 60.38
4,6 aDGlcp 98.00 71.71 72.92 69.30 73.03 60.36
4 aDGlcp 99.51 71.86 72.96 76.78 72.71 69.23
aDGlcp 99.70 71.60 73.28 76.65 71.22 60.38
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
4,4,4,4,4 aDGlcp 5.42 3.65 3.97 3.70 3.85 3.78-3.86
4,4,4,4 aDGlcp 5.42 3.65 3.97 3.70 3.85 3.78-3.86
4,4,4 aDGlcp 5.42 3.65 3.97 3.70 3.85 3.78-3.86
4,4 aDGlcp 5.42 3.65 3.97 3.70 3.85 3.78-3.86
4,6 aDGlcp 5.09 3.67 3.76 3.44 3.73 3.69-3.86
4 aDGlcp 5.40 3.61 3.73 3.68 3.75 3.45
aDGlcp 5.42 3.65 3.97 3.70 3.85 3.78-3.86
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
4,4,4,4,4 aDGlcp 99.70/5.42 71.60/3.65 73.28/3.97 76.65/3.70 71.22/3.85 60.38/3.78-3.86
4,4,4,4 aDGlcp 99.70/5.42 71.60/3.65 73.28/3.97 76.65/3.70 71.22/3.85 60.38/3.78-3.86
4,4,4 aDGlcp 99.70/5.42 71.60/3.65 73.28/3.97 76.65/3.70 71.22/3.85 60.38/3.78-3.86
4,4 aDGlcp 99.70/5.42 71.60/3.65 73.28/3.97 76.65/3.70 71.22/3.85 60.38/3.78-3.86
4,6 aDGlcp 98.00/5.09 71.71/3.67 72.92/3.76 69.30/3.44 73.03/3.73 60.36/3.69-3.86
4 aDGlcp 99.51/5.40 71.86/3.61 72.96/3.73 76.78/3.68 72.71/3.75 69.23/3.45
aDGlcp 99.70/5.42 71.60/3.65 73.28/3.97 76.65/3.70 71.22/3.85 60.38/3.78-3.86
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 4,4,4,4,4 | aDGlcp | 5.42 | 3.65 | 3.97 | 3.70 | 3.85 | 3.78
3.86 |
| 4,4,4,4 | aDGlcp | 5.42 | 3.65 | 3.97 | 3.70 | 3.85 | 3.78
3.86 |
| 4,4,4 | aDGlcp | 5.42 | 3.65 | 3.97 | 3.70 | 3.85 | 3.78
3.86 |
| 4,4 | aDGlcp | 5.42 | 3.65 | 3.97 | 3.70 | 3.85 | 3.78
3.86 |
| 4,6 | aDGlcp | 5.09 | 3.67 | 3.76 | 3.44 | 3.73 | 3.69
3.86 |
| 4 | aDGlcp | 5.40 | 3.61 | 3.73 | 3.68 | 3.75 | 3.45 |
| | aDGlcp | 5.42 | 3.65 | 3.97 | 3.70 | 3.85 | 3.78
3.86 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 4,4,4,4,4 | aDGlcp | 99.70 | 71.60 | 73.28 | 76.65 | 71.22 | 60.38 |
| 4,4,4,4 | aDGlcp | 99.70 | 71.60 | 73.28 | 76.65 | 71.22 | 60.38 |
| 4,4,4 | aDGlcp | 99.70 | 71.60 | 73.28 | 76.65 | 71.22 | 60.38 |
| 4,4 | aDGlcp | 99.70 | 71.60 | 73.28 | 76.65 | 71.22 | 60.38 |
| 4,6 | aDGlcp | 98.00 | 71.71 | 72.92 | 69.30 | 73.03 | 60.36 |
| 4 | aDGlcp | 99.51 | 71.86 | 72.96 | 76.78 | 72.71 | 69.23 |
| | aDGlcp | 99.70 | 71.60 | 73.28 | 76.65 | 71.22 | 60.38 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
(later renamed to: Cyclocybe cylindracea)