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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: fruiting body

The structure was elucidated in this paper
NCBI PubMed ID: 39521216
Publication DOI: 10.1016/j.ijbiomac.2024.137396
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: gqzhang

shzu.edu.cn
Institutions: The Key Laboratory of Oasis Agricultural Pest Management and Plant Protection Utilization, College of Agriculture, Shihezi University, Shihezi, China

A novel polysaccharide, named as PFP1-1 (23 kDa), was isolated from the fruiting body of Pleurotus ferulae. Structural analysis revealed that PFP1-1 is primarily composed of mannose, galactose, glucose and fucose, with a molar ratio of 41.50:41.92:4.65:1.93. Infrared spectroscopy analysis showed the presence of characteristic absorption peaks associated with polysaccharides. Further analysis using gas chromatography-mass spectrometry (GC-MS) and Nuclear Magnetic Resonance (NMR) indicated that the polysaccharide mainly composed of →6)-α-D-Galp-(1→,→2,6)-α-D-Galp- (1→ and a small amount of →4)-α-D-Glcp-(1→. The branched chain is mainly composed of β-D-Manp-(1→ and α-D-Glcp-(1→ connected at the O-2 position of the sugar residue →2,6)-α-D-Galp-(1→. PFP1-1 exhibited significant antifungal activity against Rhizoctonia solani and promoted cucumber plant growth. The mycelial growth inhibition rate of PFP1-1 against R. solani reached 70 %. In pot experiments, cucumber seedlings treated with PFP1-1 demonstrated resistance to R. solani infection and the incidence rate was significantly reduced to 22.92 %. PFP1-1 increased the root length and fresh weight of cucumber seedlings and enhanced the stress and disease resistance of plants by increasing the activities of superoxide dismutase, peroxidase and polyphenol oxidase. In conclusion, the present study provides a theoretical and experimental basis for the application of P. ferulae polysaccharide in promoting plant growth and controlling plant diseases

polysaccharide, Structural characterization, antifungal activity, growth-promoting, Pleurotus ferulae

Structure type: structural motif or average structure ; 23000
Location inside paper: Fig. 5, Table 4
Compound class: polysaccharide, glucogalactomannan
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_163,SB_192,SB_44,SB_7,SB_72

Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, X-ray, acid hydrolysis, biological assays, extraction, acetylation, methylation analysis, reduction, precipitation, phenol-sulfuric acid assay, SEM, sonication, Sevag method, centrifugation, antifungal activity assay, ion chromatography, FT-IR, GPC-MALLS-RI
Biological activity: Cucumber seedlings treated with compound could resist the infection of R. solani, and the incidence rate was significantly reduced, only 22.92 %. Furthermore, compound demonstrated the potential to prevent and control cucumber damping-off, caused by R. solani, and induce resistance by enhancing the activities of SOD, POD, PAL and PPO antioxidant enzymes and disease-resistant enzymes. In addition, it can also promote the growth of cucumber roots, increase fresh weight, and exhibit no harm to plants

NCBI Taxonomy refs (TaxIDs): 228215
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,4,6	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,6	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,2,4	Me	56.03
6,2	aDGlcp	98.64	71.42	71.7	78.75	70.59	61.19
6	aDGalp	98.12	76.93	70.47	69.16	71.42	68.52
6,6,6,4	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,6,6	aDGlcp	98.64	71.42	71.7	78.75	70.59	61.19
2	bDManp	101.67	70.39	72.9	69.47	72.66	61.02
	aDGalp	98.12	76.93	70.47	69.16	71.42	68.52


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,4,6	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62-3.82
6,6	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62-3.82
6,2,4	Me	3.38
6,2	aDGlcp	5.31	3.71	3.83	3.48	3.64	3.91
6	aDGalp	5.07	3.89	3.68	3.33	3.86	3.94-4.14
6,6,6,4	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62-3.82
6,6,6	aDGlcp	5.31	3.71	3.83	3.48	3.64	3.91
2	bDManp	4.74	4.04	3.57	3.85	3.68	3.69-3.85
	aDGalp	5.07	3.89	3.68	3.33	3.86	3.94-4.14


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
6,6,6,4,6	aDGalp	97.84/4.94	71.86/3.78	76.23/3.95	70.47/3.61	72.89/3.88	66.77/3.62-3.82
6,6	aDGalp	97.84/4.94	71.86/3.78	76.23/3.95	70.47/3.61	72.89/3.88	66.77/3.62-3.82
6,2,4	Me	56.03/3.38
6,2	aDGlcp	98.64/5.31	71.42/3.71	71.7/3.83	78.75/3.48	70.59/3.64	61.19/3.91
6	aDGalp	98.12/5.07	76.93/3.89	70.47/3.68	69.16/3.33	71.42/3.86	68.52/3.94-4.14
6,6,6,4	aDGalp	97.84/4.94	71.86/3.78	76.23/3.95	70.47/3.61	72.89/3.88	66.77/3.62-3.82
6,6,6	aDGlcp	98.64/5.31	71.42/3.71	71.7/3.83	78.75/3.48	70.59/3.64	61.19/3.91
2	bDManp	101.67/4.74	70.39/4.04	72.9/3.57	69.47/3.85	72.66/3.68	61.02/3.69-3.85
	aDGalp	98.12/5.07	76.93/3.89	70.47/3.68	69.16/3.33	71.42/3.86	68.52/3.94-4.14

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
6,6,6,4,6	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62 3.82
6,6	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62 3.82
6,2,4	Me	3.38
6,2	aDGlcp	5.31	3.71	3.83	3.48	3.64	3.91
6	aDGalp	5.07	3.89	3.68	3.33	3.86	3.94 4.14
6,6,6,4	aDGalp	4.94	3.78	3.95	3.61	3.88	3.62 3.82
6,6,6	aDGlcp	5.31	3.71	3.83	3.48	3.64	3.91
2	bDManp	4.74	4.04	3.57	3.85	3.68	3.69 3.85
	aDGalp	5.07	3.89	3.68	3.33	3.86	3.94 4.14

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
6,6,6,4,6	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,6	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,2,4	Me	56.03
6,2	aDGlcp	98.64	71.42	71.7	78.75	70.59	61.19
6	aDGalp	98.12	76.93	70.47	69.16	71.42	68.52
6,6,6,4	aDGalp	97.84	71.86	76.23	70.47	72.89	66.77
6,6,6	aDGlcp	98.64	71.42	71.7	78.75	70.59	61.19
2	bDManp	101.67	70.39	72.9	69.47	72.66	61.02
	aDGalp	98.12	76.93	70.47	69.16	71.42	68.52

There is only one chemically distinct structure:

*OC[C@H]1O[C@H](OC[C@H]2O[C@H](OC[C@H]3O[C@H](OC[C@H]4O[C@H](O[C@@H]5[C@@H](CO)O[C@H](OC[C@H]6O[C@H](OC[C@H]7O[C@H](OC[C@H]8O[C@H](OC[C@H]9O[C@H](OC[C@H]%10O[C@H](OC[C@H]%11O[C@H](OC[C@H]%12O[C@H](*)[C@H](O[C@@H]%13O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]%13O)[C@@H](O)[C@H]%12O)[C@H](O[C@H]%12O[C@H](CO)[C@@H](OC)[C@H](O)[C@H]%12O)[C@@H](O)[C@H]%11O)[C@H](O)[C@@H](O)[C@H]%10O)[C@H](O[C@H]%10O[C@H](CO)[C@@H](OC)[C@H](O)[C@H]%10O)[C@@H](O)[C@H]9O)[C@H](O)[C@@H](O)[C@H]8O)[C@H](O[C@H]8O[C@H](CO)[C@@H](OC)[C@H](O)[C@H]8O)[C@@H](O)[C@H]7O)[C@H](O)[C@@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@@H](O)[C@H]4O)[C@H](O)[C@@H](O)[C@H]3O)[C@H](O)[C@@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGalp: unknown unit count was assumed to be 3
Warning = sub-repeat ending at aDGalp: unknown unit count was assumed to be 3