Found 2 records.
Displayed records from 1 to 2
Expand all records
Collapse all records
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
Li JH, Gu FT, Yang Y, Zhao ZC, Huang LX, Zhu YY, Chen S, Wu JY
Simulated human digestion and fermentation of a high-molecular weight polysaccharide from Lentinula edodes mushroom and protective effects on intestinal barrier
Carbohydrate Polymers 343 (2024)
ID 122478
Lentinula edodes
(NCBI TaxID 5353,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 39174101Publication DOI: 10.1016/j.carbpol.2024.122478Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Chen S <chenshiguo210
163.com>; Wu JY <yong.wu
polyu.edu.hk>
Institutions: Research Institute for Future Food, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, Zhejiang University Shandong (Linyi) Institute of Modern Agriculture, Linyi, China
Lentinula edodes (Shiitake) is an important edible mushroom and polysaccharides are its major constituents with proven health benefits. The study was to investigate the gut bacterial fermentation and subsequent effects on gut barrier function of a glucan-rich polysaccharide, LePS40 precipitated from the mushroom water extract with 40 % (v/v) ethanol. LePS40 consisted of a β-(1→3)-glucan main chain with substitution in the C-6 position with side chains mainly composed of (1→6)-linked β-Glcp residues, (1→6)-linked α-Galp residues and terminal residues of β-Glcp. LePS40 was found highly resistant to digestive enzymes and gastric acid in simulated human gastrointestinal tract, but highly fermentable during in vitro human fecal fermentation. The fecal fermentation degradation of LePS40 appeared to selectively break the glucoside linkage in view of the dramatic decrease in the glucose molar ratio (12.68 to 1.07). Compared with the prebiotic reference FOS, LePS40 led to much higher levels of butyric, and propionic acid and a lower level of acetic acid. Moreover, LePS40 enhanced the abundance of some beneficial bacterial populations, but decreased the bacteria possibly linked with fatty-liver disease and colorectal cancer. Furthermore, the fecal fermentation products of LePS40 showed a potential protective effect on intestinal barrier function against inflammatory damage in Caco-2/Raw264.7 co-culture model. These findings suggest the potential of LePS40 for improvement of gut health through modulation of gut microbiota
polysaccharide, Intestinal barrier, Lentinula edodes, fecal microbiota fermentation, intestinal digestion
Structure type: fragment of a bigger structure
Location inside paper: p. 5, left column, paragraph 1
Trivial name: β-D-glucan
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: DNA techniques, acid hydrolysis, anion-exchange chromatography, biological assays, HPLC, acetylation, methylation analysis, reduction, dialysis, precipitation, derivatization, sonication, centrifugation, qRT-PCR, immunofluorescence, column chromatography, Lowry method, HPSEC-MALLS-RI, DNS method
Related record ID(s): 41596
NCBI Taxonomy refs (TaxIDs): 5353
Show glycosyltransferases
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Li JH, Gu FT, Yang Y, Zhao ZC, Huang LX, Zhu YY, Chen S, Wu JY
Simulated human digestion and fermentation of a high-molecular weight polysaccharide from Lentinula edodes mushroom and protective effects on intestinal barrier
Carbohydrate Polymers 343 (2024)
ID 122478
Lentinula edodes
(NCBI TaxID 5353,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 39174101Publication DOI: 10.1016/j.carbpol.2024.122478Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Chen S <chenshiguo210
163.com>; Wu JY <yong.wu
polyu.edu.hk>
Institutions: Research Institute for Future Food, Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, Zhejiang University Shandong (Linyi) Institute of Modern Agriculture, Linyi, China
Lentinula edodes (Shiitake) is an important edible mushroom and polysaccharides are its major constituents with proven health benefits. The study was to investigate the gut bacterial fermentation and subsequent effects on gut barrier function of a glucan-rich polysaccharide, LePS40 precipitated from the mushroom water extract with 40 % (v/v) ethanol. LePS40 consisted of a β-(1→3)-glucan main chain with substitution in the C-6 position with side chains mainly composed of (1→6)-linked β-Glcp residues, (1→6)-linked α-Galp residues and terminal residues of β-Glcp. LePS40 was found highly resistant to digestive enzymes and gastric acid in simulated human gastrointestinal tract, but highly fermentable during in vitro human fecal fermentation. The fecal fermentation degradation of LePS40 appeared to selectively break the glucoside linkage in view of the dramatic decrease in the glucose molar ratio (12.68 to 1.07). Compared with the prebiotic reference FOS, LePS40 led to much higher levels of butyric, and propionic acid and a lower level of acetic acid. Moreover, LePS40 enhanced the abundance of some beneficial bacterial populations, but decreased the bacteria possibly linked with fatty-liver disease and colorectal cancer. Furthermore, the fecal fermentation products of LePS40 showed a potential protective effect on intestinal barrier function against inflammatory damage in Caco-2/Raw264.7 co-culture model. These findings suggest the potential of LePS40 for improvement of gut health through modulation of gut microbiota
polysaccharide, Intestinal barrier, Lentinula edodes, fecal microbiota fermentation, intestinal digestion
Structure type: fragment of a bigger structure
Location inside paper: p. 5, left column, paragraph 1
Compound class: polysaccharide
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_167069,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: DNA techniques, acid hydrolysis, anion-exchange chromatography, biological assays, HPLC, acetylation, methylation analysis, reduction, dialysis, precipitation, derivatization, sonication, centrifugation, qRT-PCR, immunofluorescence, column chromatography, Lowry method, HPSEC-MALLS-RI, DNS method
Related record ID(s): 41595
NCBI Taxonomy refs (TaxIDs): 5353
Show glycosyltransferases
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Total list of record IDs on all result pages of the current query:
Execution: 5 sec
report error