Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 38475282Publication DOI: 10.3390/polym16050598Journal NLM ID: 101545357Publisher: Basel: MDPI
Correspondence: Zhang J <syja16
saas.sh.cn>; Zhang H <henanhaoyun
126.com>
Institutions: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, Shanghai, China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China, Jiangsu Chinagreen Biotechnology Co., Ltd., Suqian, China
FVPT1, a novel heteropolysaccharide, was purified from the fruiting body of Flammulina velutipes using magnetic-field-assisted three-phase partitioning and gel permeation chromatography. The structure was characterized using monosaccharide composition and methylation analysis, infrared spectroscopy and nuclear magnetic resonance (NMR). The FVPT1 (~16400 Da) was composed of L-fucose, D-galactose, D-glucose and D-mannose at a molar ratio of 1.0:3.5:1.0:1.4. The polysaccharide repeating unit of FVPT1 was established with methylation analyses and NMR spectroscopy. Moreover, a zebrafish larva hyperlipidemia model test demonstrated that FVPT1 can show appreciable lipid-lowering effects. In addition, the FVPT1 exhibited remarkable immunoregulatory activity by increasing nitric oxide, interleukin (IL)-1β and IL-1 secretion in macrophages. Therefore, these results suggest that FVPT1 has the potential to be developed into a new immune or hypolipidemic health product
polysaccharides, Flammulina velutipes, structural characteristics, immunoregulatory activity, lipid-lowering activity, three-phase partitioning
Structure type: structural motif or average structure ; 16400
Location inside paper: Fig. 4, Table 2, FVPT1
Compound class: polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_134624,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_190606,IEDB_983930,IEDB_983931,SB_163,SB_192,SB_31,SB_44,SB_67,SB_7,SB_72,SB_86
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, ELISA, acid hydrolysis, biological assays, GPC, HPAEC-PAD, extraction, acetylation, methylation analysis, reduction, dialysis, sonication, FT-IR, HPSEC-RI, NO determination
Biological activity: compound exhibits good immunomodulatory activity by increasing nitric oxide, interleukin (IL)-1β and IL-1 secretion in macrophages and good hypolipidemic activity via appreciable lipid-lowering effects in a zebrafish larva hyperlipidemia model test
Comments, role: NMR spectra were were removed by CSDB staff due to multiple errors in the 13C NMR assignment reported in Table 2.
NCBI Taxonomy refs (TaxIDs): 38945
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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