Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2009.04.009Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: zhouyf383
nenu.edu.cn, jmz
263.net
Institutions: School of Life Sciences, Northeast Normal University, Changchun 130024, PR China, School of Life Sciences, Changchun Normal University, Changchun 130022, PR China, College of Sciences, Shenyang Agricultural University, Shenyang 110161, PR China
A low molecular-weight polysaccharide named BIWP2 was purified from the fruit bodies of Bulgaria Inquinans (Fries) via hot-water extraction, followed by freeze-thawing and gel filtration chromatography on Sephadex G-75. Monosaccharide composition analysis revealed that BIWP2 contained exclusively glucose. High performance size exclusion chromatography (HPSEC) showed that it was a homogeneous polysaccharide fraction. Its molecular weight was estimated to be 2.6 KD and the polydispersity index (Mw/Mn) was calculated to be 1.4. Periodate oxidation, methylation, and NMR analyses indicated that BIWP2 was a linear β-(1→6)-d-glucan without side chains. This is the first time to report a linear β-(1→6)-d-glucan with low molecular weight in non-lichenized ascomycete.
polysaccharide, methylation, β-(1-6)-d-glucan, Bulgaria inquinans (Fries)
Structure type: homopolymer ; n=9; 2600
Location inside paper: p.1256, BIWP2
Trivial name: BIWP2
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, sugar analysis, GC, FTIR, HPLC, HPSEC, UV, periodate oxidation
Comments, role: water-soluble polysaccharide BIWP2
NCBI Taxonomy refs (TaxIDs): 73172Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 104.66 74.71 76.57 71.14 77.25 70.48
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.42 3.22 3.40 3.35 3.53 3.75-4.12
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 104.66/4.42 74.71/3.22 76.57/3.40 71.14/3.35 77.25/3.53 70.48/3.75-4.12
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.42 | 3.22 | 3.40 | 3.35 | 3.53 | 3.75
4.12 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 104.66 | 74.71 | 76.57 | 71.14 | 77.25 | 70.48 |
|
There is only one chemically distinct structure:
Found 
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