Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 18022685Publication DOI: 10.1016/j.ijbiomac.2007.10.010Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: aneli
uel.br (Aneli M. Barbosa)
Institutions: Dept de Bioquímica e Biotecnologia – CCE, Universidade Estadual de Londrina, C.P. 6001, C.E.P. 86051-990, Londrina-PR, Brazil, Universidad de Castilla - La Mancha, Instituto de Regional Investigación Científica Aplicada (IRICA), 13071 Cuidad Real, Spain, Dept de Biologia Geral – CCB, Universidade Estadual de Londrina, C.P. 6001, C.E.P. 86051-990, Londrina-PR, Brazil
Biopolymers such as exopolysaccharides (EPS) are produced by microbial species and possess unusual properties known to modify biological responses, among them are antimutagenicity and immunomodulation. Botryosphaeran, a newly described fungal (1 → 3; 1 → 6)-β-D-glucan produced by Botryosphaeria rhodina MAMB-05, was administered by gavage to mice at three doses (7.5, 15 and 30 mg/kg b.w.per day) over 15 days, and found to be non-genotoxic by the micronucleus test in peripheral blood and bone marrow. Botryosphaeran administered at doses of 15 and 30 mg EPS/kg b.w. decreased significantly (p < 0.001) the clastogenic effect of cyclophosphamide-induced micronucleus formation resulting in a reduction of the frequency of micronucleated cells of 78 and 82% in polychromatic erythrocytes of bone marrow, and reticulocytes in peripheral blood, respectively. The protective effect was dose-dependent, and strong anticlastogenic activity was exerted at low EPS doses. Variance analysis (ANOVA) showed no significant differences (p < 0.05) among the median body weights of the groups of mice treated with botryosphaeran during experiments evaluating genotoxic and protective activities of botryosphaeran. This is the first report on the biological activity attributed to botryosphaeran.
mouse, Botryosphaeran, Botryosphaeria rhodina MAMB-05, Cyclophosphamide, Anticlastogenicity, Micronucleus test
Structure type: polymer chemical repeating unit
Location inside paper: p.173
Trivial name: botryosphaeran
Compound class: EPS, glucan, polysaccharide
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assays, extraction, statistical analysis, dialysis, cytological analyses, micronucleus test
Biological activity: Botryosphaeran was found to be non-genotoxic by the micronucleus test in peripheral blood and bone marrow; it decreased the clastogenic effect of cyclophosphamide-induced micronucleus formation resulting in a reduction of the frequency of micronucleated cells in polychromatic erythrocytes of bone marrow, and reticulocytes in peripheral blood, respectively.
Related record ID(s): 48304, 48981, 49980, 50037, 50070, 50624, 50629, 50630, 50631
NCBI Taxonomy refs (TaxIDs): 45133Reference(s) to other database(s): GTC:G59945WE
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There is only one chemically distinct structure:
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(later renamed to: Lasiodiplodia theobromae MAMB-05)