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Cryptococcus neoformans ATCC H99

(previously named: Cryptococcus neoformans A H99; later renamed to: Cryptococcus neoformans var. grubii H99)
(NCBI TaxID 235443, species name lookup)

Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Associated disease: infection due to Cryptococcus neoformans [ICD11: XN3EH

]

NCBI PubMed ID: 20070311
Publication DOI: 10.1111/j.1462-5822.2010.01430.x
Publisher: New York: J. Wiley & Sons Ltd
Editors: Soldati T, Gow N, Grinstein S, Hartland E, Locker JK, Scherf A, Shao F
Correspondence: nosanchu

aecom.yu.edu
Institutions: Departments of Medicine (Division of Infectious Diseases) and Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY, USA

The polysaccharide capsule of the fungus Cryptococcus neoformans is its main virulence factor. In this study, we determined the effects of mannitol and glucose on the capsule and exopolysaccharide production. Growth in mannitol significantly increased capsular volume compared with cultivation in glucose. However, cells grown in glucose concentrations higher than 62.5 mM produced more exopolysaccharide than cells grown in mannitol. The fibre lengths and glycosyl composition of capsular polysaccharide from yeast grown in mannitol was structurally different from that of yeast grown in glucose. Furthermore, mannitol treatment of mice infected intratracheally with C. neoformans resulted in fungal cells with significantly larger capsules and the mice had reduced fungal dissemination to the brain. Our results demonstrate the capacity of carbohydrate source and concentration to modify the expression of a major virulence factor of C. neoformans. These findings may impact the clinical management of cryptococcosis.

capsular polysaccharide, mannitol, Cryptococcus neoformans, cryptococcosis

Structure type: structural motif or average structure
Location inside paper: p.740
Compound class: CPS, galactoxylomannan (GalXM)
Contained glycoepitopes: IEDB_114701,IEDB_130701,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_144983,IEDB_145668,IEDB_151528,IEDB_152206,IEDB_164174,IEDB_167188,IEDB_174332,IEDB_190606,IEDB_983930,SB_163,SB_165,SB_166,SB_187,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: GC-MS, ELISA, composition analysis, light scattering, statistical analysis, zeta potential measurement, fluorescence-activated cell sorter analysis
Comments, role: The structure is described ambiguously in the paper. Corrected using branched component of galactoxylomannan (GalXM) from [Doering TL, 2000] and [Vaishnav VV, 1998].

Related record ID(s): 41714, 42147
NCBI Taxonomy refs (TaxIDs): 235443
Reference(s) to other database(s): GTC:G16353MJ
Show glycosyltransferases

There is only one chemically distinct structure:

[*]OC[C@H]1O[C@H](OC[C@H]2O[C@H]([*])[C@H](O)[C@@H](O)[C@H]2O)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@@H]5OC[C@@H](O)[C@H](O)[C@H]5O)[C@@H]4O)[C@@H]3O[C@@H]3OC[C@@H](O)[C@H](O)[C@H]3O)[C@H](O[C@@H]3OC[C@@H](O)[C@H](O)[C@H]3O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize