Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)
Organ / tissue: cell wall
 
Publication DOI: 10.1007/s13213-014-1019-4
Journal NLM ID: 100959580
Publisher: Milano, Italy: University of Milan, Dept. of Food Science and Microbiology
Correspondence: Al-Askar AA <alaskaraksu.edu.sa>; Baka ZA <zakariabakayahoo.com>; Rashad YM <younesrashadyahoo.com>; Ghoneem KM <khalid_ghoneemyahoo.com>; Abdulkhair WM <waleed_hamadayahoo.com>; Shabana YM <yassershabana2yahoo.com>; Hafez EE <elsayed_hafezyahoo.com>
Institutions: Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia, Botany Department, College of Science, Damietta University, Damietta, Egypt, Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Alexandria, Egypt, Department of Seed Pathology Research, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt, Microbiology Department, General Department of Basic Medical Sciences, National Organization for Drug Control and Research, Giza, Egypt, Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

Antagonistic actinomycete strains isolated from the environment are valuable tools for an eco-friendly, healthy, and safe control of phytopathogenic fungi. We have evaluated the culture filtrate of Streptomyces griseorubens E44G, an actinomycete strain isolated from soil, on the growth and ultrastructure of hyphal cells of the phytopathogenic fungus Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt disease of tomato. The effect of the Streptomyces culture filtrate on some of the carbohydrate fractions in the hyphal cell of the pathogen using gold-labeled lectin complexes was also elucidated. Of the concentrations of S. griseorubens E44G culture filtrate tested, the highest (400 mu L) had the most potent antifungal effect on the mycelial growth of the fungus. At this concentration, some changes in the morphology of the fungal hyphae were observed by scanning electron microscopy, and a number of dramatic changes in the ultrastructure of the hyphal cells of the fungus were observed by transmission electron microscopy. Ultracytochemical localization of carbohydrate fractions of the hyphal cell of the fungus revealed the presence of a very high quantity of chitin in the cell wall which was digested following exposure to the culture filtrate of S. griseorubens E44G, indicating the presence of a chitinase enzyme in that filtrate. The ultracytochemical investigations also indicated the presence of mannose, glucose, and galactose in the fungal cell wall, as well as the absence of glucosides. Moreover, the fungal cell cytoplasm contained glucosides and galactose but not chitin. These results confirm that the chitinase enzyme was produced by S. griseorubens E44G and that this enzyme may play a role in the potential of this strain as an antifungal agent against F. oxysporum f. sp. lycopersici.

antagonist, TEM, chitinase, SEM, sugar localization, ultracytochemical

Structure type: polymer chemical repeating unit
Location inside paper: p. 1823, column 1, paragraph 1
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
 
Methods: SEM, TEM, ultracytochemical studies
Comments, role: OTI: fibrillar core of the cell wall
 
Related record ID(s): 42301
NCBI Taxonomy refs (TaxIDs): 746128
Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
 

Convert structure to SMILES & 3D GlycoCT β-test WURCS 2.0 GLYCAM GlycoCT (external) GlycoCT XML (ext) GlydeII XML LinUCS Fragmentize Mol. weight

Simulate NMR spectra (GODDESS)

Show Sweet-II 3D model Generate 3D coords by GLYCAM