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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_131174,IEDB_133754,IEDB_135610,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_143254,IEDB_151531,IEDB_225177,IEDB_423153,IEDB_885823

• Article ID: 1580
  Vinogradov EV, Brade L, Brade H, Holst O "The structure of the O-specific polysaccharide of the lipopolysaccharide from Acinetobacter strain 44 (DNA group 3)" - Polish Journal of Chemistry 79 (2005) 267-273
  

  Extraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses and NMR spectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was: [see formula in the text]. After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG3 isotype) was obtained which reacted in Western blot with this LPSZ and characterized it as S-form.

  Lipopolysaccharide, structure, DNA, strain, polysaccharide, repeating unit, group, Acinetobacter, O-polysaccharide, O polysaccharide, O-specific, O-specific polysaccharide, tyvelose, Enterobacter

  Journal NLM ID: 7901356
  WWW link: http://www.ichf.edu.pl/pjch/pj-2005/pj-2005-02c.pdf
  Publisher: Państwowe Wydawnictwo Naukowe
  Institutions: Divisions of Biochemical Microbiology, Structural Biochemistry, Research Center Borstel, Leibniz Center for Medicine and Biosciences, D-23845 Borstel, Germany
  Methods: NMR, Smith degradation, composition analysis
  
   
  
  Acinetobacter sp. 44 (DNA group 3)
  CSDB ID 10181 (all data & tools)
• Article ID: 4329
  Knirel YA "Structure of O-antigens" - Book: Bacterial lipopolysaccharides: Structure, chemical synthesis, biogenesis and interaction with host cells (2011) Chapter 3, 41-115
  

  The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is designated as the O-specific polysaccharide (O-polysaccharide, OPS) or O-antigen. The O-antigen is the most variable portion of the LPS and provides serological specificity, which is used for bacterial serotyping. The OPS also provides protection to the microorganisms from host defenses such as complement mediated killing and phagocytosis, and is involved in interactions of bacteria with plants and bacteriophages. Studies of the OPSs ranging from the elucidation of their chemical structures and conformations to their biological and physico-chemical properties help improving classification schemes of Gram-negative bacteria. Furthermore, these studies contributed to a better understanding of the mechanisms of pathogenesis of infectious diseases, as well as provided information to develop novel vaccines and diagnostic reagents.

  Lipopolysaccharide, synthesis, lipopolysaccharides, structure, Bacterial, host, O-antigen, O antigen, cell, O antigens, O-antigens, chemical, interaction, cells, PDF, chemical synthesis, biogenesis

  Publication DOI: 10.1007/978-3-7091-0733-1_3
  Publisher: Springer
  Correspondence: knirel@ioc.ac.ru
  Editors: Knirel YA, Valvano MA
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  
   
  
  Acinetobacter sp. 44
  CSDB ID 27756 (all data & tools)