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Structure type: oligomer
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_135610,IEDB_135813,IEDB_136105,IEDB_137340,IEDB_141807,IEDB_151531,IEDB_225177,IEDB_885823

• Article ID: 1580
  Vinogradov EV, Brade L, Brade H, Holst O "The structure of the O-specific polysaccharide of the lipopolysaccharide from Acinetobacter strain 44 (DNA group 3)" - Polish Journal of Chemistry 79 (2005) 267-273
  

  Extraction of dry bacteria of Acinetobacter strain 44 (DNA group 3) by phenol/water gave a polymer that was identified by means of serological studies as S-form lipopolysaccharide (LPS). Mild acetic acid hydrolysis degraded the O-specific polysaccharide, thus, its structure was investigated by compositional analyses and NMR spectroscopy after de-O-acylation and Smith degradation of the LPS. The structure of the O-specific polysaccharides was: [see formula in the text]. After immunization of BALB/c mice with Acinetobacter strain 44, monoclonal antibody S48-26 (IgG3 isotype) was obtained which reacted in Western blot with this LPSZ and characterized it as S-form.

  Lipopolysaccharide, structure, DNA, strain, polysaccharide, repeating unit, group, Acinetobacter, O-polysaccharide, O polysaccharide, O-specific, O-specific polysaccharide, tyvelose, Enterobacter

  Journal NLM ID: 7901356
  WWW link: http://www.ichf.edu.pl/pjch/pj-2005/pj-2005-02c.pdf
  Publisher: Państwowe Wydawnictwo Naukowe
  Institutions: Divisions of Biochemical Microbiology, Structural Biochemistry, Research Center Borstel, Leibniz Center for Medicine and Biosciences, D-23845 Borstel, Germany
  Methods: NMR, Smith degradation, composition analysis
  
   
  
  Acinetobacter sp. 44 (DNA group 3)
  CSDB ID 10464 (all data & tools)

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Structure type: oligomer
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_137472,IEDB_141794,IEDB_144983,IEDB_152206,IEDB_190606,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 3573
  Zdorovenko EL, Varbanets LD, Zatonsky GV, Kachala VV, Zdorovenko GM, Shashkov AS, Knirel YA "Structure of the O-specific polysaccharide of the lipopolysaccharide of Rahnella aquatilis 95 U003" - Carbohydrate Research 343(14) (2008) 2494-2497
  

  The O-polysaccharide of Rahnella aquatilis 95 U003 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [carbohydrate structure: see text].

  Lipopolysaccharide, O-antigen, bacterial polysaccharide structure, Rahnella aquatilis

  NCBI PubMed ID: 18674754
  Publication DOI: 10.1016/j.carres.2008.07.001
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: evelina@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, RussiaD.K. Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, ul. Zabolotnogo 154, 03143 Kiev, Ukraine
  Methods: 13C NMR, 1H NMR, methylation, NMR-2D, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, NMR-1D
  
   
  
  Rahnella aquatilis 95 U003
  CSDB ID 23347 (all data & tools)