Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 26076647Publication DOI: 10.1016/j.carbpol.2015.05.034Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Farinha I <isf17210
campus.fct.unl.pt>; Duarte P <paulo.duarte.neves
gmail.com>; Pimentel A <acgp
campus.fct.unl.pt>; Plotnikova E <janepostbox
gmail.com>; Chagas B <bf.chagas
campus.fct.unl.pt>; Mafra L <lmafra
ua.pt>; Grandfils C <C.Grandfils
ulg.ac.be>; Freitas F <a4406
fct.unl.pt>; Fortunato E <emf
fct.unl.pt>; Reis MA <amr
fct.unl.pt>
Institutions: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, Caparica, Portugal, CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa and CEMOP-UNINOVA, Campusde Caparica, Caparica, Portugal, Interfacultary Research Centre of Biomaterials (CEIB), University of Liège, Liège, Belgium, CICECO, Department of Chemistry, University of Aveiro, Aveiro, Portugal
Purified chitin-glucan complex (CGCpure) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The co-polymer thus obtained had a β-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGCpure had an average molecular weight of 4.9 × 10(5)Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGCpure has α-chitin in its structure. CGCpure presented an endothermic decomposition peak at 315°C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products.
structural analysis, molecular weight, chitin–glucan complex (CGC), Komagataella pastoris, thermal properties
Structure type: homopolymer
Location inside paper: p. 459, column 2, paragraph 4, Fig. 3(left)
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, callose, laminarin, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, cell wall glucan, polysaccharide, glycoside, β-glucan, β-1, 3-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: FTIR, HPLC, SEC, alkaline treatment, centrifugation, X-ray diffraction, HCl hydrolysis, Lowry method, TFA hydrolysis, gravimetric analysis, pyrolysis, 13C CPMAS NMR
Comments, role: chitin-glucan complex
3D data: conformation data
Related record ID(s): 42367
NCBI Taxonomy refs (TaxIDs): 4922Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.5 74.2-76.4 85.7 68.5 74.2-76.4 62.0
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.5 | 74.2
76.4 | 85.7 | 68.5 | 74.2
76.4 | 62.0 |
|
There is only one chemically distinct structure:
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