Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 23544515Publication DOI: 10.1016/j.carbpol.2012.12.073Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Van Griensven LJ <leo.vangriensven
wur.nl>; Van Griensven LJ <leo.vangriensven
gmail.com>
Institutions: Wageningen University and Research Centre, Wageningen, The Netherlands, Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil, Plant Research International, Wageningen University and Research, Wageningen, The Netherlands
The (1→6)-β-D-glucans from Agaricus bisporus and Agaricus brasiliensis were purified to evaluate their effects on the innate immune system. THP-1 macrophages were used to investigate the induction of the expression of TNF-α, IL1β, and COX-2 by RT-PCR. The purification of the polysaccharides gave rise to fractions containing 96-98% of glucose. The samples were analyzed by GC-MS, HPSEC and (13)C NMR, which confirmed the presence of homogeneous (1→6)-β-D-glucans. The β-glucans were incubated with THP-1 derived macrophages, for 3 h and 6 h to evaluate their effects on the expression of pro-inflammatory genes. Both β-glucans stimulated the expression of such genes as much as the pro-inflammatory control (LPS). When the cells were incubated with LPS+β-glucan, a significant inhibition of the expression of IL-1β and COX-2 was observed for both treatments after 3 h of incubation. By the results, we conclude that the (1→6)-β-D-glucans present an immunostimulatory activity when administered to THP-1 derived macrophages.
Immunostimulatory activity, Agaricus bisporus, (1→6)-β-D-glucan, Agaricus brasiliensis
Structure type: homopolymer ; 29000-45000
Location inside paper: Fig. 3, Fig. 2, Glc-Ab, Glc-Abz
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, methylation, GC-MS, HPSEC, extraction, acetylation, NaBH4 reduction, dialysis, centrifugation, TFA hydrolysis, precipitaion, cytokine assays
Biological activity: after treatment of the human moncytic cell line THP-1 with isolated glucans it was concluded that they present an immunostimulatory activity; The b-glucan from A. bisporus (Glc-Ab) was able to reduce or inhibit the expression of IL-1β to 40%, at 50 μg/mL, after 3 h of treatment, compared to the pro-inflammatory control (LPS); Expression of COX-2 was reduced 50% or more, after 3 h of treatment for all doses; The β-glucan from A. brasiliensis (Glc-Abz) also significantly reduced or inhibited the expression of IL-1β and COX-2 compared to the pro-inflammatory control: The highest dose of β-glucan showed a reduction of 64% for IL-1β, and 36% for COX-2; The concentration of 50 μg/mL reduced the expression of COX-2 by 59% compared to the pro-inflammatory control
Comments, role: molecular masses: Glc-Ab - 29000; Glc-Abz - 45000
Related record ID(s): 48766
NCBI Taxonomy refs (TaxIDs): 5341,
307931Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 323(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.1 73.3 76.4-76.5 69.8-70.0 75.3-75.4 68.3-68.4
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.1 | 73.3 | 76.4
76.5 | 69.8
70.0 | 75.3
75.4 | 68.3
68.4 |
|
There is only one chemically distinct structure:
Found 
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