Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 26691385Publication DOI: 10.1016/j.ijbiomac.2015.12.014Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Wang Q <wangqiang06
caas.cn>
Institutions: Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agriculture Sciences, Beijing, China, State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China, Food Science Department, Cornell University, Ithaca, USA
In order to enhance the solubility of yeast β-D-glucan and enlarge the applicable area, we investigated dynamic high pressure microfluidization (DHPM) treatment with ionic liquids as solvent to prepare water-soluble yeast β-D-glucan (WSG). The solubility of β-D-glucan was greatly impacted by the pressure of DHPM, residence time, and ratio of β-D-glucan and EmimAc. The yield of WSG reached 79.25 % (w/w) with the water solubility of 72.50 % (w/w). The DHPM treatment did not destroy the ionic liquid structure therefore the ionic liquids could be recycled. FTIR and (13)C NMR spectrum results showed that WSG was the polysaccharides with (1→3)-β-D-glycosidic bonds, after modification, the hydrogen bonding was destroyed. The chain conformation of WSG in 0.1M NaNO3 aqueous solutions at 25 °C were studied by laser light scattering and viscometry. The Mark-Houwink equation was established to be [η]=2.09×10(-2) MW(0.63) (cm(3)g(-1)). According to the conformational parameters calculated from wormlike cylinder model, WSG existed as semi-stiff chain in aqueous solution.
structure, solubilization, yeast β-D-glucan
Structure type: structural motif or average structure ; 1240000 (F1), 407000 (F2), 170000 (F3), 110000 (F4), 42300 (F4), 26100 (F6)
Location inside paper: water-soluble yeast β-D-glucan, WSG, Table 2, Table 3
Compound class: β-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FTIR, HPSEC-MALLS, viscosity measurement, extraction, sulfuric acid hydrolysis
Comments, role: WSG was prepared from yeast β-D-glucan by dynamic high pressure microfluidization (DHPM) treatment with ionic liquids as solvent; WSG is presented by polysaccharide fractions with β-1,3-glycosidic bonds, compared with yeast β-D-glucan, replacements on C-6 were found in WSG according to 13C NMR data discussion (see section 3.4.2, p. 399)
3D data: conformation data
Related record ID(s): 42513
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 358(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.08 72.88 86.25 68.44 76.37 60.91
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.08 | 72.88 | 86.25 | 68.44 | 76.37 | 60.91 |
|
There is only one chemically distinct structure:
Found 
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