Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 2736561Publication DOI: 10.1016/0008-6215(89)84040-6Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Agricultural Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan, Institute of Laboratory Animals, Mie University School of Medicine, Mie, Japan, Department of Pharmacology, Mie University School of Medicine, Mie, Japan, Nichirei Research Institute, Tokyo, Japan
Some polysaccharide-containing materials were successively extracted from the fruiting bodies of Agaricus blazei with aqueous ammonium oxalate and sodium hydroxide, fractionated, and assayed for antitumor activity. From chemical analyses and n.m.r. data, it was concluded that the most active fraction, FIII-2-b, was comprised of protein and a (1→6)-β-D-glucan.
polysaccharide, glucan, Agaricus blazei, fruiting body
Structure type: homopolymer ; 10000-50000
Location inside paper: FIII-2-b, (1→6)-β-D-glucan, Scheme 1 (FIII-2-b), fig. 1, fig. 2
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, 1H NMR, IR, GLC-MS, GLC, optical rotation measurement, acetylation, methylation analysis, reduction with NaBH4, phenol-sulfuric acid assay, antitumor activity assay, COSY, HCl hydrolysis, sulfuric acid hydrolysis
Biological activity: SFA1 polysaccharide exhibited anti-tumor activity against sarcoma 180 with inhibition ratio of 99.1% at 10 mg/kg dose
Comments, role: NMR temperature was not specificied. The value for the protein content of FIII-2-b was 43.3%.
NCBI Taxonomy refs (TaxIDs): 79798Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O / NaOD
[as TSV]
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.47 3.29 3.40 3.40 3.57 3.82-4.28
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.47 | 3.29 | 3.40 | 3.40 | 3.57 | 3.82
4.28 |
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Found 
report error