Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 27392770Publication DOI: 10.1016/j.ijbiomac.2016.07.016Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Gao Q <czxuduoduo
163.com>
Institutions: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin, China, Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin, China, Departments of Ophthalmology, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin, China, Preparation Center, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin, China
A water-soluble polysaccharide (EPA-1) from Pleurotus eryngii was obtained using DEAE-52 and Sephadex G-50 columns. The properties, structure and immunomodulatory activity of EPA-1 were studied. The results demonstrated that EPA-1 was a homogeneous polysaccharide with the molecular weight of 9.97*10^4 Da. EPA-1 consisted of Man, Glc and Gal in a molar ratio of 2.2:1.0:3.2. The characterized fragment structures of EPA-1 were found to be consisting of seven sugar residues and two branches by GC-MS, FTIR and NMR analyses. Among them, the (1→6)-linkedGal residue was the main linkage mode of EpA-1 and composed of its backbone. Activity tests indicated that EPA-1 significantly induced macrophage to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κΒ, indicating EPA-1 to possess good immunoregulatory activity.
polysaccharide, cytokine, immunostimulation, Pleurotus eryngii, MAPKs, NF-κB
Structure type: fragment of a bigger structure ; n=5
Location inside paper: EPA-1, fig.3
Compound class: mannogalactan
Contained glycoepitopes: IEDB_130701,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153217,IEDB_153218,IEDB_156489,IEDB_156493,IEDB_156557,IEDB_164174,IEDB_190606,IEDB_742248,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: 13C NMR, 1H NMR, IR, GC-MS, HPLC, UV, statistical analysis, methylation analysis, cell viability assay, macrophage activity assay, phenol-sulfuric acid assay, nitric oxide assay, COSY HSQC
Biological activity: EPA-1 significantly induced RAW 264.7 macrophage cells to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κB (see Fig. 4).
Comments, role: NMR temperature was not specificied; part of proposed EPA-1 polysaccharide complex stucture, which consists of successively linked ID 42843 and ID 42844; full structure is 99.7 kDa
Related record ID(s): 42844
NCBI Taxonomy refs (TaxIDs): 5323Reference(s) to other database(s): GTC:G11374FA
Show glycosyltransferases
NMR conditions: in D2O
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 27392770Publication DOI: 10.1016/j.ijbiomac.2016.07.016Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Gao Q <czxuduoduo
163.com>
Institutions: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin, China, Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin, China, Departments of Ophthalmology, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin, China, Preparation Center, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin, China
A water-soluble polysaccharide (EPA-1) from Pleurotus eryngii was obtained using DEAE-52 and Sephadex G-50 columns. The properties, structure and immunomodulatory activity of EPA-1 were studied. The results demonstrated that EPA-1 was a homogeneous polysaccharide with the molecular weight of 9.97*10^4 Da. EPA-1 consisted of Man, Glc and Gal in a molar ratio of 2.2:1.0:3.2. The characterized fragment structures of EPA-1 were found to be consisting of seven sugar residues and two branches by GC-MS, FTIR and NMR analyses. Among them, the (1→6)-linkedGal residue was the main linkage mode of EpA-1 and composed of its backbone. Activity tests indicated that EPA-1 significantly induced macrophage to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κΒ, indicating EPA-1 to possess good immunoregulatory activity.
polysaccharide, cytokine, immunostimulation, Pleurotus eryngii, MAPKs, NF-κB
Structure type: fragment of a bigger structure ; n=2
Location inside paper: EPA-1, fig. 3
Compound class: mannogalactan
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_165,SB_166,SB_187,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: 13C NMR, 1H NMR, IR, GC-MS, HPLC, UV, statistical analysis, methylation analysis, cell viability assay, macrophage activity assay, phenol-sulfuric acid assay, nitric oxide assay, COSY HSQC
Biological activity: EPA-1 significantly induced RAW 264.7 macrophage cells to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κB (see Fig. 4).
Comments, role: NMR temperature was not specificied; part of proposed EPA-1 polysaccharide complex stucture, which consists of successively linked ID 42843 and ID 42844; full structure is 99.7 kDa
Related record ID(s): 42843
NCBI Taxonomy refs (TaxIDs): 5323Reference(s) to other database(s): GTC:G16355EG
Show glycosyltransferases
NMR conditions: in D2O
1H NMR data: present in publication
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
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