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Jiang P, Yuan L, Huang G, Wang X, Li X, Jiao L, Zhang L
Structural properities and immunoenhancement of an exopolysaccharide produced by Phellinus pini
International Journal of Biological Macromolecules 93(A) (2016)
566-571
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a-D-Manp-(1-3)-a-D-Manp-(1-6)-+
|
-2)-a-D-Manp-(1-2)-a-D-Manp-(1- |
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Phellinus pini
(later renamed to: Porodaedalea pini)
(NCBI TaxID 108901,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 27612645Publication DOI: 10.1016/j.ijbiomac.2016.09.020Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Jiao L <jianganji
hotmail.com>; Zhang L <zhanglp625
163.com>
Institutions: School of Life Sciences, Northeast Normal University, Changchun, China, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China, Agricultural and Animal Husbandry College of Tibet University, Xizang, China, Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, China
Phellinus pini exopolysaccharide (PPE) is a mannan produced by P. pini when cultured in potato dextroseagar medium. Partial hydrolysis with acid, periodate oxidation and Smith degradation, methylation and 13C nuclear magnetic resonance (NMR) spectroscopy were used to elaborate its structure. Results indicated that PPE contained a backbone of (1→2)-linked Man, which was heavily substituted via (1→6)-glucosidic bonds with (1→3)-linked Man, and terminated mainly with Man, as well as a small amount of Gal and Glc. Immunological tests showed that PPE exhibited high macrophages-activating ability, thereby augmenting phagocytosis and enhancing the production of nitric oxide, tumour necrosis factor-α and reactive oxygen species. Therefor, PPE had potential applications in developing functional food or in serving as a natural immnunostimulant.
polysaccharides, macrophage, Phellinus pini
Structure type: structural motif or average structure
Location inside paper: fig. 3. PPE, fig. 2
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141793,IEDB_141795,IEDB_141830,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_164174,IEDB_164480,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_198,SB_44,SB_67,SB_72,SB_77
Methods: 13C NMR, GC-MS, GC, Smith degradation, HPLC, periodate oxidation, acetylation, statistical analysis, methylation analysis, reduction with NaBH4, macrophage activity assay, phagocytosis assay, TNF-a assay, nitric oxide assay, TFA hydrolysis, reduction with KBH4
Biological activity: Effect of different doses of PPE on NO (μM), TNF-α (ng/mL) and ROS (% of control) production at effective concentration of 200 μg/mL: 33.45± 3.84 μM, 402.15± 11.34 ng/mL and 179.77±17.63%, respectively.
NCBI Taxonomy refs (TaxIDs): 108901Reference(s) to other database(s): GTC:G80360GV
Show glycosyltransferases
NMR conditions: in D2O at 298(C) K
[as TSV]
13C NMR data: present in publication
|
There is only one chemically distinct structure:
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Liu Y, You Y, Zeng S, Hu Y, Duan X, Liu A, Chen H, Hu X, Chen S, Li C, Chen D
Structural characterization and antidiabetic activity of a glucopyranose-rich heteropolysaccharide from Catathelasma ventricosum
Carbohydrate Polymers 149 (2016)
399-407
Catathelasma ventricosum
(NCBI TaxID 181977,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 27261764Publication DOI: 10.1016/j.carbpol.2016.04.106Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Chen D <dwchen
sicau.edu.cn>
Institutions: Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China, College of Food Science, Sichuan Agricultural University, Yaan, China
Polysaccharides, which are the main bioactive constituents of edible mushrooms, have been shown to have a variety of useful biological activities. In this study, a polysaccharide fraction from the edible mushroom Catathelasma ventricosum was purified using anion exchange and size exclusion chromatographies. The structure of the resulting polysaccharide, named CVP-1S, was characterized on the basis of partial acid hydrolysis, periodic acid oxidation, methylation analysis, gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, 1D/2D NMR spectroscopy, scanning electron microscopy, and atomic force spectroscopy. The results showed that CVP-1S is a heteropolysaccharide consisting of glucose (94.2%), galactose (3.51%) and fucose (1.3%) with a molecular weight of 1.5*10^4 Da. Its backbone is mainly linked by (1→6)-β-D-Glcp glycosidic bonds, and branches are attached to the backbone through 1,3-linked glycosidic bonds. CVP-1S was also found to have antioxidant, hypoglycemic, and hypolipidemic activities in the streptozoicin-induced diabetic mouse model. From these results, we conclude that CVP-1S should receive further attention as a potential agent for the treatment and prevention of diabetes.
polysaccharide, structure characterization, antidiabetic activity, Catathelasma ventricosum
Structure type: homopolymer ; 15000
Location inside paper: CVP-1S, fig. 3
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, HPAEC, GC, Smith degradation, FTIR, biochemical methods, HPLC, UV, periodate oxidation, acetylation, statistical analysis, methylation analysis, reduction with NaBH4, antioxidant activities, HPGPC, SEM, COSY, HSQC, hypoglycemic activity assay, TFA hydrolysis, reduction with KBH4
Biological activity: CVP-1S was also found to have antioxidant, hypoglycemic, and hypolipidemic activities in the streptozoicin-induced diabetic mouse model (see Tables 2-4).
Comments, role: published NMR chemical shifts of #_bDGlcp C4 and C5 that seemed erroneously interchanged in the published assignment were swapped by CSDB staff
NCBI Taxonomy refs (TaxIDs): 181977Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.12 73.23 75.78 69.71 75.07 69.036
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.57 3.37 3.54 3.68 3.90 4.26
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.12/4.57 73.23/3.37 75.78/3.54 69.71/3.68 75.07/3.90 69.036/4.26
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.57 | 3.37 | 3.54 | 3.68 | 3.90 | 4.26 |
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13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.12 | 73.23 | 75.78 | 69.71 | 75.07 | 69.036 |
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There is only one chemically distinct structure:
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Cheng H, Jia Y, Wang L, Liu X, Liu G, Li L, He C
Isolation and structural elucidation of a novel homogenous polysaccharide from Tricholoma matsutake
Natural Product Research 30(1) (2016)
58-64
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D-Fucp-(1-2)-D-Manp-(1-3)-a-D-Galp-(1-6)-+
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-4)-b-D-Glcp-(1-4)-b-D-Glcp-(1- |
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Tricholoma matsutake
(NCBI TaxID 40145,
species name lookup)
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 26208132Publication DOI: 10.1080/14286419.2015.1034711Journal NLM ID: 101167924Publisher: Milton Park, UK : Taylor & Francis Health Sciences
Correspondence: He C <congfenhe
126.com.cn>
Institutions: Beijing Key Laboratory of Plants Resource Research and Development, School of Science, Beijing Technology and Business University, Beijing, China, Infinitus International (China) Company, Ltd., Guangzhou, Guangdong, China
A crude polysaccharide possessing antitumour, radiation-resistant and anti-ageing attributes was extracted from Tricholoma matsutake by water extraction and alcohol precipitation. From this crude polysaccharide, a homogeneous polysaccharide, TMP-5II, was successfully purified by Sephacryl S-300 column chromatography. The average molecular weight (Mw) of TMP-5II was 15.76 kDa. Monosaccharide analysis indicated that the homogeneous polysaccharide contained four different residues: d-glucose, d-galactose, d-mannose and d-fucose. Attenuated total reflectance infrared spectroscopy revealed characteristics typical of carbohydrate polymers and a peak typical of a β-type glycosidic bond. TMP-5II was selected for structural characterisation by nuclear magnetic resonance (NMR) analysis. According to (1)H NMR, (13)C NMR and two-dimensional-NMR analysis, TMP-5II contains two kinds of linkages, β and α, at a ratio of 4:1. Preliminary results indicated that the polysaccharide had (1-4)-beta-pyran glucose as the main chain, and a branched chain in the O-6 location with fucose (1-2) mannose (1-3)-alpha-pyran galactose.
polysaccharide, structure elucidation, 2D-NMR, GC-MS, Tricholoma matsutake
Structure type: structural motif or average structure ; 15760
Location inside paper: fig. S6, fig. S7, fig. 1, TMP-5II
Contained glycoepitopes: IEDB_115015,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140529,IEDB_141794,IEDB_142488,IEDB_142489,IEDB_144983,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_152206,IEDB_167069,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72,SB_86
Methods: 13C NMR, 1H NMR, GC-MS, FTIR, HPLC, acetylation, HMBC, HSQC, GPC-MALLS, TFA hydrolysis, reduction with KBH4
NCBI Taxonomy refs (TaxIDs): 40145Reference(s) to other database(s): GTC:G43334OK
Show glycosyltransferases
NMR conditions: in D2O at 303 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
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