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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)
Organ / tissue: mycelium

The structure was elucidated in this paper
Publication DOI: 10.1016/j.jtice.2016.05.052
Journal NLM ID: 101550026
Publisher: Amsterdam, Taipei: Elsevier; Dept. of Chemical Engineering, National Taiwan University
Correspondence: Yan JK <jkyan27

mail.ujs.edu.cn>
Institutions: School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China

In this study, a water-soluble polysaccharide (PLP1-I) was purified from Phellinus linteus mycelia through hot water extraction, ethanol precipitation, and chromatography on DEAE Sepharose Fast Flow and Sephacryl S-400 HR columns. PLP1-I was characterized as a neutral heteropolysaccharide composed of glucose and galactose at a molar ratio of 8.9:1.0, with an average molecular weight (Mw) of ~290,000 kDa. The structure of PLP1-I consists of a (1→4)-α-D-glucopyranose backbone, whereas the (1→3)-α-D-galactopyranosyl residues formed branches at the O-6 position with 1-linked-α-D-glucopyranosyl terminal residues. Evaluation of the antioxidant activity in vivo showed that PLP1-I administration significantly increased the activity of antioxidant enzymes and reduced the levels of malondialdehyde in the serum and liver of aging mice induced with D-galactose. PLP1-I also effectively stimulated the immune system of aging mice. This study demonstrated that PLP1-I exhibited strong antioxidant activities in vivo and could be explored as a potential antioxidant for functional food and medicine.

polysaccharide, purification, Antioxidant activity, Phellinus linteus, structural characteristics

Structure type: structural motif or average structure ; 290000000
Location inside paper: PLP1-I, fig. 3, fig. 4
Compound class: glucan
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_140629,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192,SB_7

Methods: 13C NMR, GC-MS, GC, FTIR, biochemical methods, UV, optical rotation measurement, acetylation, statistical analysis, reduction with NaBH4, antioxidant activities, HPGPC, phenol-sulfuric acid assay, TFA hydrolysis, methyaltion analysis, IH NMR
Biological activity: In vivo antioxidant assay showed that PLP1-I significantly enhanced the activities of antioxidant enzymes (SOD, GSH-Px, and CAT) and clearly reduced the levels of MDA in the serum and liver of aging mice. PLP1-I also effectively stimulated the immune function of aging mice (see Tables 3-5).

NCBI Taxonomy refs (TaxIDs): 1659895
Show glycosyltransferases

NMR conditions: in D2O at 298 K

¹H NMR data: present in publication

¹³C NMR data: present in publication

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](CO)O[C@H](O[C@@H]2[C@@H](CO)O[C@H](O[C@@H]3[C@@H](CO)O[C@H](O[C@@H]4[C@@H](CO)O[C@H](O[C@@H]5[C@@H](CO)O[C@H](O[C@@H]6[C@@H](CO)O[C@H](O[C@@H]7[C@@H](CO)O[C@H](O[C@@H]8[C@@H](O)[C@@H]([*])O[C@H](CO[C@H]9O[C@H](CO)[C@@H](O)[C@H](O)[C@H]9O)[C@@H]8O)[C@H](O)[C@H]7O)[C@H](O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGlcp: large unit count (6) was reduced to 5