Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
The structure was elucidated in this paperNCBI PubMed ID: 27261764Publication DOI: 10.1016/j.carbpol.2016.04.106Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Chen D <dwchen
sicau.edu.cn>
Institutions: Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China, College of Food Science, Sichuan Agricultural University, Yaan, China
Polysaccharides, which are the main bioactive constituents of edible mushrooms, have been shown to have a variety of useful biological activities. In this study, a polysaccharide fraction from the edible mushroom Catathelasma ventricosum was purified using anion exchange and size exclusion chromatographies. The structure of the resulting polysaccharide, named CVP-1S, was characterized on the basis of partial acid hydrolysis, periodic acid oxidation, methylation analysis, gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, 1D/2D NMR spectroscopy, scanning electron microscopy, and atomic force spectroscopy. The results showed that CVP-1S is a heteropolysaccharide consisting of glucose (94.2%), galactose (3.51%) and fucose (1.3%) with a molecular weight of 1.5*10^4 Da. Its backbone is mainly linked by (1→6)-β-D-Glcp glycosidic bonds, and branches are attached to the backbone through 1,3-linked glycosidic bonds. CVP-1S was also found to have antioxidant, hypoglycemic, and hypolipidemic activities in the streptozoicin-induced diabetic mouse model. From these results, we conclude that CVP-1S should receive further attention as a potential agent for the treatment and prevention of diabetes.
polysaccharide, structure characterization, antidiabetic activity, Catathelasma ventricosum
Structure type: homopolymer ; 15000
Location inside paper: CVP-1S, fig. 3
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, HPAEC, GC, Smith degradation, FTIR, biochemical methods, HPLC, UV, periodate oxidation, acetylation, statistical analysis, methylation analysis, reduction with NaBH4, antioxidant activities, HPGPC, SEM, COSY, HSQC, hypoglycemic activity assay, TFA hydrolysis, reduction with KBH4
Biological activity: CVP-1S was also found to have antioxidant, hypoglycemic, and hypolipidemic activities in the streptozoicin-induced diabetic mouse model (see Tables 2-4).
Comments, role: published NMR chemical shifts of #_bDGlcp C4 and C5 that seemed erroneously interchanged in the published assignment were swapped by CSDB staff
NCBI Taxonomy refs (TaxIDs): 181977Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 103.12 73.23 75.78 69.71 75.07 69.036
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.57 3.37 3.54 3.68 3.90 4.26
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 103.12/4.57 73.23/3.37 75.78/3.54 69.71/3.68 75.07/3.90 69.036/4.26
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.57 | 3.37 | 3.54 | 3.68 | 3.90 | 4.26 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 103.12 | 73.23 | 75.78 | 69.71 | 75.07 | 69.036 |
|
There is only one chemically distinct structure:
Found 
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