The structure was determined for the capsular polysaccharide (CPS) isolated from a swarming strain of Proteus vulgaris, CP2-96, which was obtained from the spleen of an infected mouse. The CPS was extracted from the cell pellet by hot water, precipitated with ethanol, and further purified by gel-permeation chromatography. The structure was established by glycosyl composition and linkage analyses, and by NMR spectroscopy. The sequence of the glycosyl residues was determined by a NOESY experiment. The CPS is composed of a tetrasaccharide repeating unit with the following structure: →4)-β-D-Glcp-(1→3)-β-D-GalpNAc4OAc-(1→2)-α-D-Glcp-(1→4)-α-D-GlcpA-(1→.

Lipopolysaccharide, structure, capsular polysaccharide, polysaccharides, Proteus vulgaris, swarming

← originally published structure   Show legend

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
4,2,3	bDGlcp	105.2	73.9	77.1	81.8	75.7	61.9
4,2,2	Ac
4,2,4	Ac
4,2	bDGalpN	104.3	52.8	81.2	69.2	76.1	61.7
4	aDGlcp	99.3	79.3	72.8	70.9	72.6	61.9
	aDGlcpA	101.2	72.6	74.8	77.6	74.6	?


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,2,3	bDGlcp	4.53	3.36	3.77	3.61	3.60	3.73-3.91
4,2,2	Ac
4,2,4	Ac
4,2	bDGalpN	4.72	4.03	3.92	4.19	3.72	3.76
4	aDGlcp	5.63	3.61	3.76	3.41	3.71	3.80-3.85
	aDGlcpA	5.32	3.67	3.98	3.81	4.08	-


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
4,2,3	bDGlcp	105.2/4.53	73.9/3.36	77.1/3.77	81.8/3.61	75.7/3.60	61.9/3.73-3.91
4,2,2	Ac
4,2,4	Ac
4,2	bDGalpN	104.3/4.72	52.8/4.03	81.2/3.92	69.2/4.19	76.1/3.72	61.7/3.76
4	aDGlcp	99.3/5.63	79.3/3.61	72.8/3.76	70.9/3.41	72.6/3.71	61.9/3.80-3.85
	aDGlcpA	101.2/5.32	72.6/3.67	74.8/3.98	77.6/3.81	74.6/4.08	

The spectrum also has 1 signal at unknown position (not plotted).

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

[*]O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](NC(C)=O)[C@H](O[C@@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@@H]3[C@@H](C(=O)O)O[C@H]([*])[C@H](O)[C@H]3O)O[C@H](CO)[C@@H]2OC(C)=O)[C@H](O)[C@H]1O

745.637 g/mol (C₂₈H₄₃R₂NO₂₂, R = next and previous repeats, not counted in mol weight)

The structure was determined for the capsular polysaccharide (CPS) isolated from a swarming strain of Proteus vulgaris, CP2-96, which was obtained from the spleen of an infected mouse. The CPS was extracted from the cell pellet by hot water, precipitated with ethanol, and further purified by gel-permeation chromatography. The structure was established by glycosyl composition and linkage analyses, and by NMR spectroscopy. The sequence of the glycosyl residues was determined by a NOESY experiment. The CPS is composed of a tetrasaccharide repeating unit with the following structure: →4)-β-D-Glcp-(1→3)-β-D-GalpNAc4OAc-(1→2)-α-D-Glcp-(1→4)-α-D-GlcpA-(1→.

Lipopolysaccharide, structure, capsular polysaccharide, polysaccharides, Proteus vulgaris, swarming

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

[*]O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](O[C@@H]2[C@@H](NC(C)=O)[C@H](O[C@@H]3[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]3O[C@@H]3[C@@H](C(=O)O)O[C@H]([*])[C@H](O)[C@H]3O)O[C@H](CO)[C@@H]2OC(C)=O)[C@@H]1O

745.637 g/mol (C₂₈H₄₃R₂NO₂₂, R = next and previous repeats, not counted in mol weight)