Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: mycelium
The structure was elucidated in this paperNCBI PubMed ID: 4085071Publication DOI: 10.1248/cpb.33.3395Journal NLM ID: 0377775Publisher: Pharmaceutical Society Of Japan
Institutions: Tokyo College of Pharmacy, Tokyo, Japan, Nippon Beet Sugar Mfg. Co., Ltd, Tokyo, Japan
The fruit body of Grifola frondosa, an edible mushroom, is used as a food in Japan. However, the matted mycelium of the fruit body is not utilized, In this paper, structural characterization and antitumor activity of the extracts from the matted mycelium of this fungus were examined. Hot water, cold alkali, and hot alkali extracts of the mycelium all contained polysaccharides ; that in the cold alkali extract was composed of glucose, but those in the hot water and hot alkali extracts contained glucose, mannose and/or xylose. Each fraction showed potent antitumor activity against murine solid tumor, Sarcoma-180. The antitumor activities of the alkali extracts were more potent than that of the hot water extract. By means of DEAE-Sephadex chromatography, α-amylase treatment, and SP-Sephadex chromatography, a neutral β-1, 3-glucan was purified from the cold alkali extract. From the results of nuclear magnetic resonance spectroscopy, methylation, periodate oxidation, enzymic degradation, and physicochemical characterizations, the purified antitumor glucan was concluded to be a β-1, 3-glucan branched at C-6 of every third main-chain glucosyl unit. The structure is quite similar to that of the antitumor glucan obtained from the fruit body of this fungus. It is concluded that the matted mycelium of G. frondosa may be useful as a source of antitumor β-1,3-glucan.
β-1, 3-glucan, antitumor activity, Grifola frondosa, matted mycelium
Structure type: polymer chemical repeating unit ; 750000
Location inside paper: Fig. 3, sec. Abstract & Discussion, grifolan NMF-5N
Trivial name: Grifolan LE
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, TLC, Smith degradation, HPLC, enzymatic digestion, periodate oxidation, methylation analysis, reduction with NaBH4, Congo Red assay
Biological activity: 20 g/mL against sarcoma 180 tumor cells with 94% inhibition ratio due to activation of macrophages and T cells
Comments, role: C6-branched branched β-1,3-glucan at every 3 residues
Related record ID(s): 43069, 43071, 43074
NCBI Taxonomy refs (TaxIDs): 5627Reference(s) to other database(s): GTC:G66305IS
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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