The partial structure and immunology of the lipopolysaccharide (LPS) of Pseudomonas stutzeri KMM 226, a bacterium isolated from a seawater sample collected at a depth of 2000 m, was characterised. The O-polysaccharide was built up of disaccharide repeating units constituted by L-Rhap and D-GlcpNAc: →2)-α-L-Rhap-(1→3)-α-D-GlcpNAc-(1→. The structural analysis of the lipid A showed a mixture of different species. The major species were hexa-acylated and penta-acylated lipids A, bearing the 12:0(3-OH) in amide linkage and 10:0(3-OH) in ester linkage, while the secondary fatty acids were present only as 12:0. The presence of 12:0(2-OH) was not detected. The immunology experiments demonstrated that P. stutzeri KMM 226 LPS displayed a low ability to induce TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine production and acted as an antagonist of hexa-acylated Escherichia coli LPS in human blood in vitro.
Lipopolysaccharide, lipid A, O-polysaccharide, antagonist, Pseudomonas stutzeri, Marine deep-sea environment
NCBI PubMed ID: 28668995Publication DOI: 10.1007/s10482-017-0907-6Journal NLM ID: 0372625Publisher: Dordrecht: Kluwer Academic
Correspondence: maxchem@mail.ru
Institutions: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Prospect 100 let Vladivostoku, 159, 100-letiya Vladivostoka prosp, Vladivostok, Russian Federation, 690022, Far Eastern Federal University, Suhanova St., 8, Vladivostok, Russian Federation, 690950
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, ELISA, ESI-MS, mild acid hydrolysis, GC, MS/MS, composition analysis, methanolysis, GPC, acetylation, statistical analysis, cytokine production
Found