Zhang H, Nie S, Cui SW, Xu M, Ding H, Xie M Characterization of a bioactive polysaccharide from Ganoderma atrum: re-elucidation of the fine structure Carbohydrate Polymers158 (2017)
58-67
/Variants 0/-+
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-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-
/Variants 0/ is:
b-D-Glcp-(1-4)-b-D-GlcpA-(1-6)-
OR (exclusively)
{{{-b-D-Glcp-(1-6)-}}}b-D-Glcp-(1-6)-
OR (exclusively)
b-D-Glcp-(1-4)-b-D-GlcA-(1-6)-{{{-b-D-Glcp-(1-6)-}}}b-D-Glcp-(1-6)-
Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota) Organ / tissue:fruiting body
The structure was elucidated in this paper NCBI PubMed ID:28024543 Publication DOI:10.1016/j.carbpol.2016.11.088 Journal NLM ID:8307156 Publisher: Elsevier Correspondence: Nie S <spniencu.edu.cn>; Xie M <myxiencu.edu.cn> Institutions: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China, Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Canada
The fine structure in terms of backbone and branch chain features of a bioactive polysaccharide from Ganoderma atrum (PSG-1) was re-elucidated systematically using high performance anion-exchange chromatography (HPAEC), methylation and GLC-MS analysis, and 1D & 2D NMR spectroscopy. Monosaccharide composition analysis revealed that PSG-1-F0.2 fraction mainly consisted of glucose (73.8%) and glucuronic acid (15.3%), with small amount of mannose (5.7%) and galactose (5.2%). Based on methylation, multistep partial acid hydrolysis and NMR study, were proposed to substitute at the O-6 position of β-(1→3)-glucan. The small amount of mannose and galactose residues were considered to be from the other fraction in PSG which was very difficult to be separated from PSG-1-F0.2. This revised structure as an acidic β-(1→3, 1→6)-glucan is considered to be more accurate than the previous proposal of PSG-1.
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ncu.edu.cn>; Xie M <myxie