Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
Publication DOI: 10.1021/ma60078a019Journal NLM ID: 0365316Publisher: ACS
Institutions: Department of Macromolecular Science, Osaka University, Osaka, Japan
A native sample of a Schizophyllum commune polysaccharide (schizophyllan) and its sonicated fragments, ranging in weight-average molecular weight (Mw)fr om 100000 to 6000000 g/mol, were studied by ultracentrifugation and viscometry in water at 25 C, in which schizophyllan has been found to exist as a triple helix. Measurements of intrinsic viscosity [η] and limiting sedimentation coefficient so showed that the schizophyllan triple helix is almost perfectly rigid up to Mw = 500000 g/mol but acquires flexibility for higher Mw. The data in the lower molecular weight range were analyzed by Yamakawa’s theory for [η] and Yamakawa-Fujii’s theory for so of a long rigid cylinder, with the result that ML (the molar mass per unit cylinder length) = 2150±150 nm-1 and d (the diameter) = 2.6 ± 0.4 nm. This ML gives the pitch of the triple helix per β-1,3-D-glucose residue a value of 0.30 ± 0.02 nm, which agrees closely with the reported pitches for lentinan and a β-1,3-D-Xylan in the crystalline region. The d value 2.6 nm is consistent with the diameter of the model triple helix for schizophyllan. With ML = 2150 ± 150 nm-1 and d = 2.6 ± 0.4 nm, the persistence length that allows the theoretical curves of so and [η] for wormlike cylinders to fit the present data was found to be 200 ± 30 nm. This value indicates that the schizophyllan triple helix is stiffer than that of native collagen, a known triple-helical biopolymer.
polysaccharide, β-glucan, beta-glucan
Structure type: polymer chemical repeating unit
Location inside paper: p.1462
Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan, schizophyllan, scleroglucan, pleuran
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, scleroglucan, D-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: ultracentrifugation, sedimentation analysis, viscosity measurment
Related record ID(s): 41391, 41610, 41614, 42637, 42640, 43382, 43400, 49988, 50026, 50061, 50064, 50072, 50089
NCBI Taxonomy refs (TaxIDs): 5334Reference(s) to other database(s): GTC:G66305IS, CCSD:
49943, CBank-STR:12679, CA:9050-67-3
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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