CSDB: Search results

Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

Publication DOI: 10.1016/j.carbpol.2011.04.075
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: Methacanon P <pawadeem

mtec.or.th>
Institutions: National Metal and Materials Technology Center (MTEC), Klong Luang, Thailand, National Center for Genetic Engineering and Biotechnology (BIOTEC), Klong Luang, Thailand

Since many studies reveal that the biological properties of various biopolymers depend on their molecular weight. Therefore, it was considered of importance to investigate how molecular weight affected on the interleukin-8 (IL-8) stimulating activity of the β-glucan from Ophiocordyceps dipterigena BCC2073. γ-Irradiation of the glucan with various doses (0-100 kGy) was chosen as a clean method to produce different low molecular weight samples. The result showed that average molecular weight (MW) of irradiated samples significantly decreased as the irradiation dose increased whereas the functional groups of before and after irradiated glucans detected by FTIR and 13C-NMR were identical. However, difference in intensity of an absorption peak at 270 nm was found in the UV/vis spectra. The biological properties such as cytotoxicity, proliferation and IL-8 secretion of normal human dermal fibroblast contacted with various MW glucans were also tested. It was found that the glucan with MW of approximately 5 kDa exhibited the highest ability to induce IL-8 production. Apart from the MW effect, chain conformation seems to be involved. Thus, differences in solution conformation of various MW glucans via Congo red analysis were evaluated.

molecular weight, Chain conformation, γ-irradiation, fungal glucan, il-8

Structure type: structural motif or average structure ; 590000
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558867,IEDB_558869,IEDB_857743,IEDB_983931,SB_192

Methods: 13C NMR, ELISA, FTIR, GPC, UV, cytotoxicity assay, precipitation, phenol-sulfuric acid assay, MTT, Congo red assay, gamma irradiation, interleukin-8 assay
Biological activity: sample irradiated at 100 kGy possessing the lowest molecular weight of 5.2 kDa apparently stimulated the fibroblasts to produce the highest IL-8 content (2.38 ng/ml); samples irradiated between 0 and 50 kGy with molecular weight ranging from 120 to 9.2 kDa stimulated the fibroblasts to produce IL-8 about 0.73–1.28 ng/ml; molecular weight range the IL-8 production is not directly proportional to molecular weight; IL-8 content gradually raised to a maximum value at molecular weight of 35 kDa (at 10 kGy) and then dropped to the lowest value at molecular weight of 9.5 kDa (at 50 kGy)
Comments, role: γ-irradiation was shown to be an effective method to produce various low-molecular weight β-glucan without noticeable changes in functional groups of the glucan
3D data: conformation data

Related record ID(s): 41263, 43492
NCBI Taxonomy refs (TaxIDs): 235378
Reference(s) to other database(s): GTC:G77421BL
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@H]1[C@H](O)[C@@H](CO[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)O[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@@H]3[C@@H](O)[C@H](O[C@@H]4[C@@H](O)[C@H](O[C@@H]5[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]5O)O[C@H](CO[C@@H]5O[C@H](CO[C@@H]6O[C@H](CO[C@@H]7O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@@H](O)[C@H](O)[C@H]6O)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)O[C@H](CO[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@H]3O)O[C@H](CO)[C@H]2O)[C@@H]1O

SVG MW SMILES 3D mol Ionize