Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 18667302Publication DOI: 10.1016/j.biortech.2008.06.036Journal NLM ID: 9889523Publisher: Elsevier
Correspondence: Sun Y <yongxusun1978
yahoo.com.cn>; Liu J <jichengliu
yahoo.cn>
Institutions: Department of Pharmacology, Qiqihar Medical University, Qiqihar, China
The water-soluble polysaccharide (POP), with a molecular mass of 24000 Da, was obtained from the fruiting body of Pleurotus ostreatus. Structure features of the purified polysaccharide were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, Smith degradation, GC-MS, 13C and 1H NMR and FTIR. The results indicated that the backbone of POP was composed of (1→6)-linked-α-d-galactopyranosyl and (1→2,6)-linked-α-d-galactopyranosyl residues, which were terminated with a single terminal (1→)-β-D-glucose residue at the O-2 position of galactosyl along the main chain in the ratio of 1:1:1. Preliminary tests in vitro showed POP is capable of enhancing concanavalin A (ConA)- or lipopolysaccharide (LPS)-induced lymphocyte proliferation, which suggested that POP could be a potential immunostimulating agent for use in functional foods or medicine against both pathogens and cancer.
polysaccharide, structural analysis, NMR spectroscopy, lymphocyte proliferation, Pleurotus ostreatus
Structure type: structural motif or average structure ; 24000
Location inside paper: Fig.1, POP, table 2
Compound class: O-polysaccharide, glucogalactan
Contained glycoepitopes: IEDB_134624,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_163,SB_192,SB_7
Methods: 13C NMR, 1H NMR, methylation, IR, GC-MS, ELISA, acid hydrolysis, GC, GPC, HPSEC, UV, ion-exchange chromatography, extraction, ethanol precipitation, limphocyte proliferation assay
Biological activity: POP could increase the ConA or LPS-induced lymphocytes proliferation in vitro, while CPOP (crude polysaccharide) has no stimulating effects on murine lymphocyte proliferation which can be explained by immunosuppressive substances that may exist in the crude polysaccharide
Related record ID(s): 50055
NCBI Taxonomy refs (TaxIDs): 5322Reference(s) to other database(s): GTC:G24814NP
Show glycosyltransferases
There is only one chemically distinct structure:
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