Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 7172167Publication DOI: 10.1016/0008-6215(82)85027-1Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Gifu College of Pharmacy, Gifu, Japan
A (1→6)-branched (1→3)-β-D-glucan (T-5-N), isolated from a sodium hydroxide extract of the fruit bodies of Dictyophora indusiata Fisch., markedly exhibited anti-inflammatory effects on both carrageenan-induced edema and scalded edematous hyperalgesia in rat's hindpaws. The activities of T-5-N (25 mg/kg i.p. × 2) were more potent than those of phenylbutazone (25-50 mg/kg i.p. × 2). The conformational behavior of T-5-N was studied. Its molecular weight in neutral solution was about three times that in 0.25m sodium hydroxide. This finding, in addition to the results of optical rotatory measurement and complex-formation with Congo Red, indicated that T-5-N has an ordered, triple-helical structure in neutral or slightly alkaline solution (< 0.15m NaOH), and has single chains in highly alkaline solution (0.25m NaOH). The conformational transition occurs at concentrations of sodium hydroxide in the range of 0.15-0.25m.
glucan, Dictyophora indusiata, anti-inflammatory agent, carrageenan
Structure type: structural motif or average structure ; 1000000
Location inside paper: introduction, paragraph 1, T-5-N
Compound class: O-polysaccharide, glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: acid hydrolysis, biological assays, GPC, viscosity measurement, extraction, optical rotation measurement, complex-formation with Congo Red
Biological activity: T-5-N exhibits significant anti-inflammatory activity and inhibits scalded edematous hyperalgesia in rat's paws, and in both cases the effect was stronger than that of phenylbutazone
Related record ID(s): 44042, 44045, 44055, 49105
NCBI Taxonomy refs (TaxIDs): 146777Reference(s) to other database(s): GTC:G83138WS
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There is only one chemically distinct structure:
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