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Taxonomic group: fungi / Ascomycota (Phylum: Ascomycota)

The structure was elucidated in this paper
Publication DOI: 10.1016/S0922-338X(97)83583-0
Journal NLM ID: 9000935
Institutions: Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Kagawa, Japan

Fusarium and Giberella species were found to secrete acidic polysaccharides when grown in Czapex-Dox medium. Fusarium oxysporum showed the highest rate of secretion of acidic sugars into the growth medium. The acidic sugar-containing glycoprotein from F. oxysporum was purified from the culture filtrate to apparent homogeneity on ultracentrifugation analysis, by ammonium sulfate precipitation, Toyopearl HW-55 gel filtration and charcoal chromatography. The purified acidic sugar-containing glycoprotein has a 10% protein content and a content of 40% acidic sugars as glucuronic acid, and the neutral sugars present in this glycoprotein are mainly mannose, galactose, and glucose. The protein moiety of the glycoprotein contains a high proportion of serine and threonine residues. Treatment of the glycoprotein with alkaline solution resulted in an increase in absorbance at 241 nm, and alkaline borohydride treatment resulted in a marked decrease in the number of serine and threonine residues. We conclude that the glycoprotein has O-linked sugar chains which are mainly attached to serine and threonine residues of the protein moiety. The primary structure of the acidic polysaccharide from F. oxysporum was analyzed mainly by NMR spectrometry. The main parts of this polysaccharide have structures similar to those of uronic acid-containing polysaccharide from Fusarium sp. M7-1.

acidic polysaccharide, glucuronic acid, glycoprotein, Fusarium

Structure type: structural motif or average structure ; 59250
Location inside paper: Fig.1, A, p.199
Compound class: O-polysaccharide, glucuronogalactomannan
Contained glycoepitopes: IEDB_115136,IEDB_133966,IEDB_136095,IEDB_137472,IEDB_137485,IEDB_140630,IEDB_142488,IEDB_144983,IEDB_144995,IEDB_144998,IEDB_146664,IEDB_149176,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72

Methods: 1H NMR, acid hydrolysis, amino acid analysis, HPLC, alkaline hydrolysis, enzymatic digestion, reduction, CC, cell growth, phenol-sulfuric acid assay, ultracentrifugation, Lowry method, m-hydroxydiphenyl colorymetry method
Enzymes that release or process the structure: lyase
Comments, role: Cellulomonas lyase was used for enzymatic digestion

NCBI Taxonomy refs (TaxIDs): 5507
Show glycosyltransferases

NMR conditions: in D2O at 343(H) K [as TSV]

13C NMR data:
missing...

¹H NMR data: present in publication

There are 6 chemically distinct structures. Please, select:

*OC[C@@H](O)[C@@H]1O[C@@H](OC[C@@H](O)[C@@H]2O[C@@H](OC[C@@H](O)[C@@H]3O[C@@H](OC[C@@H](O)[C@@H]4O[C@@H](*)[C@H](O[C@H]5O[C@H](C(=O)O)[C@@H](O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]6O)[C@H](O)[C@H]5O)[C@H]4O)[C@H](O[C@H]4O[C@H](C(=O)O)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]5O)[C@H](O)[C@H]4O)[C@H]3O)[C@H](O[1*])[C@H]2O)[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize

R1 = unidentified oligosaccharide