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Found 3 records.
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1. (CSDB ID: 44111) | report error |
| -6)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1- | Show graphically |
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Aspergillus fumigatus CBS 143-89
(Ancestor NCBI TaxID 746128,
species name lookup)
]The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate. Nuclear magnetic resonance and gas-liquid chromatography-mass spectrometry analysis have been performed on intact GM, acid-hydrolyzed GM, and oligomers resulting from the acetolysis of the acid-hydrolyzed GM. Results show that A. fumigatus GM is composed of a linear mannan core with an α-(1-2)-linked mannotetraose repeating unit attached via α-(1-6) linkage. Side chains composed of an average of 4 to 5 β-(1-5)-galactofuranose units are linked to C-6 and C-3 positions of α-(1-2)-linked mannose units of the mannan. The immunoreactivity of GM and HCl- hydrolyzed GM was studied by use of human sera from aspergillosis patients and an antigalactofuran monoclonal antibody. The α-(1-2)(1-6)-mannan core is not antigenic. The immunogenic galactofuran is found amongst several exocellular glycoproteins. According to a direct enzyme-linked immunosorbent assay with GM as the detector antigen, only 26% of the serum samples from aspergilloma patients (all positive by immunodiffusion assays) give optical density values superior to a cutoff estimated as the mean ±3 standard deviations of values obtained with control sera.
immunogenicity, Galactomannan, Aspergillus fumigatus, aspergilloma
Structure type: structural motif or average structure ; n=9-1013C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 2,2,2 aDManp 104.8 73.4 74.5 69.2 75.6 68.5 2,2 aDManp 103.3-103.4 80.7-80.8 73.0-73.1 69.9-70.0 76.1 63.7 2 aDManp 103.3-103.4 80.7-80.8 73.0-73.1 69.9-70.0 76.1 63.7 aDManp 103.1 81.2 72.8 69.7 76.0 64.0 1H NMR data: missing...
13C NMR data:
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2. (CSDB ID: 44112) | report error |
| a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp-(1-2)-a-D-Manp | Show graphically |
|
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Aspergillus fumigatus CBS 143-89
(Ancestor NCBI TaxID 746128,
species name lookup)
]The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate. Nuclear magnetic resonance and gas-liquid chromatography-mass spectrometry analysis have been performed on intact GM, acid-hydrolyzed GM, and oligomers resulting from the acetolysis of the acid-hydrolyzed GM. Results show that A. fumigatus GM is composed of a linear mannan core with an α-(1-2)-linked mannotetraose repeating unit attached via α-(1-6) linkage. Side chains composed of an average of 4 to 5 β-(1-5)-galactofuranose units are linked to C-6 and C-3 positions of α-(1-2)-linked mannose units of the mannan. The immunoreactivity of GM and HCl- hydrolyzed GM was studied by use of human sera from aspergillosis patients and an antigalactofuran monoclonal antibody. The α-(1-2)(1-6)-mannan core is not antigenic. The immunogenic galactofuran is found amongst several exocellular glycoproteins. According to a direct enzyme-linked immunosorbent assay with GM as the detector antigen, only 26% of the serum samples from aspergilloma patients (all positive by immunodiffusion assays) give optical density values superior to a cutoff estimated as the mean ±3 standard deviations of values obtained with control sera.
immunogenicity, Galactomannan, Aspergillus fumigatus, aspergilloma
Structure type: oligomer13C NMR data: Linkage Residue C1 C2 C3 C4 C5 C6 2,2,2 aDManp 104.8 73.1 72.8 69.6 75.9 63.9 2,2 aDManp 103.2 81.0-81.2 72.7 69.8 75.9 63.8 2 aDManp 103.2 81.0-81.2 72.7 69.8 75.9 63.8 aDManp 95.2 81.8 72.7 69.8 75.2 63.8 1H NMR data: missing...
13C NMR data:
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3. (CSDB ID: 44113) | report error |
| {{{-b-D-Galf-(1-5)-}}}/n=4-5/-b-D-Galf-(1-3)-+ | {{{-b-D-Galf-(1-5)-}}}/n=4-5/-b-D-Galf-(1-6)-+ | | | -2)-a-D-Manp-(1-2)-a-D-Manp-(1-6)-a-D-Manp-(1-2)-a-D-Manp-(1- | Show graphically |
|
Show legend Show as text |
Aspergillus fumigatus CBS 143-89
(Ancestor NCBI TaxID 746128,
species name lookup)
]The galactomannan (GM) produced extracellularly by Aspergillus fumigatus has been purified by a double sequential hydrazine-nitrous acid treatment of the ethanol precipitate of the culture filtrate. Nuclear magnetic resonance and gas-liquid chromatography-mass spectrometry analysis have been performed on intact GM, acid-hydrolyzed GM, and oligomers resulting from the acetolysis of the acid-hydrolyzed GM. Results show that A. fumigatus GM is composed of a linear mannan core with an α-(1-2)-linked mannotetraose repeating unit attached via α-(1-6) linkage. Side chains composed of an average of 4 to 5 β-(1-5)-galactofuranose units are linked to C-6 and C-3 positions of α-(1-2)-linked mannose units of the mannan. The immunoreactivity of GM and HCl- hydrolyzed GM was studied by use of human sera from aspergillosis patients and an antigalactofuran monoclonal antibody. The α-(1-2)(1-6)-mannan core is not antigenic. The immunogenic galactofuran is found amongst several exocellular glycoproteins. According to a direct enzyme-linked immunosorbent assay with GM as the detector antigen, only 26% of the serum samples from aspergilloma patients (all positive by immunodiffusion assays) give optical density values superior to a cutoff estimated as the mean ±3 standard deviations of values obtained with control sera.
immunogenicity, Galactomannan, Aspergillus fumigatus, aspergilloma
Structure type: structural motif or average structure ; n=9-10| New query | Export IDs | Home | Help |
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