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Taxonomic group: fungi / Basidiomycota (Phylum: Basidiomycota)

The structure was elucidated in this paper
Publication DOI: 10.1016/j.carbpol.2009.04.036
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: ekhong

kangwon.ac.kr
Institutions: Department of Bioengineering and Technology, Kangwon National University, Chuncheon, South Korea, School of Bioscience and Biotechnology, Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, South Korea

Recently, a number of bioactive molecules, including antitumor agents, have been identified in various higher basidiomycetes mushrooms. Polysaccharides are the best known and most potent mushroom derived substances that display immuno-pharmacological properties. In this study, the water soluble crude polysaccharide HEB-P, which was obtained from the liquid culture broth of Hericium erinaceus by ethanol precipitation, was fractionated by DEAE cellulose and Sepharose CL-6B column chromatography. This fractionation process resulted in two polysaccharide fractions that were termed HEB-NP Fr I and HEB-AP Fr I. Of the fractions, HEB-AP Fr I was able to upregulate the functional events mediated by activated macrophages, such as production of nitric oxide (NO) and expression of cytokines (IL-1β and TNF-α). Its structural characteristics were investigated by a combination of chemical and instrumental analyses, including methylation, reductive cleavage, acetylation, Fourier transform infrared spectroscopy (FT-IR), and gas chromatography-mass spectrometry (GC-MS). Results indicate that HEB-AP Fr I was a low molecular mass polysaccharide with a laminarin-like triple helix conformation of the β-1,3-branched-β-1,2-mannan.

Hericium erinaceus, immunostimulating polysaccharide, laminarin-like triple helix, macrophage activation

Structure type: structural motif or average structure ; 46000
Location inside paper: Table 3, HEB-AP Fr I
Compound class: O-polysaccharide, mannan
Contained glycoepitopes: IEDB_115576,IEDB_131173,IEDB_133966,IEDB_133967,IEDB_134618,IEDB_137485,IEDB_140116,IEDB_144983,IEDB_144995,IEDB_152206,IEDB_1539315,IEDB_173895,IEDB_76920,IEDB_858578,IEDB_983930,SB_44,SB_72

Methods: methylation, gel filtration, IR, GC-MS, ELISA, acid hydrolysis, HPLC, UV, extraction, RT-PCR, reduction, CC, cell growth, determination of NO production, cell viability assay, ethanol precipitation, Bradford method, evaporation, Congo Red assay, phenol–sulfuric acid assay, macrophage production, glucose assay, Elson–Morgan method, Blumenkrantz method
Biological activity: HEB-AP Fr I very effectively upregulates cytokine expression (TNF-a and IL-1b) and NO release indicating that it was able to induce the functional activation of macrophages

Related record ID(s): 44122
NCBI Taxonomy refs (TaxIDs): 91752
Reference(s) to other database(s): GTC:G53199UW
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@H]([*])O[C@H](CO[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@@H]4O)[C@@H]3O)[C@@H]2O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: bacteria, algae / Proteobacteria, Ochrophyta (Phylum: Proteobacteria, Ochrophyta)

Publication DOI: 10.1016/j.carbpol.2009.04.036
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: ekhong

Hericium erinaceus, immunostimulating polysaccharide, laminarin-like triple helix, macrophage activation

Structure type: homopolymer
Location inside paper: Fig.6, A, curdlan, laminarin
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192

Methods: methylation, gel filtration, IR, GC-MS, ELISA, acid hydrolysis, HPLC, UV, extraction, RT-PCR, reduction, CC, cell growth, determination of NO production, cell viability assay, ethanol precipitation, Bradford method, evaporation, Congo Red assay, phenol–sulfuric acid assay, macrophage production, glucose assay, Elson–Morgan method, Blumenkrantz method

Related record ID(s): 44121
NCBI Taxonomy refs (TaxIDs): 511, 80365
Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:157, CCSD:50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](O)[C@H]([*])O[C@H](CO)[C@H]1O

SVG MW SMILES 3D mol Ionize