Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 6240491Publication DOI: 10.1093/oxfordjournals.jbchem.a134917Journal NLM ID: 0376600Publisher: Japanese Biochemical Society
Institutions: Department of Agricultural Chemistry, Tohoku University, Sendai, Japan
The native proteoheteroglycan (PHG) from mycelia of Neurospora crassa contains two kinds of carbohydrate chains differing structure. The oligosaccharides containing mannose and galactofuransoe are attached by O-glycosidic linkages to serine or threonine residues in the protein (J. Biochem. 96, 1005-1011, 1984). The second kind of carbohydrate chain is a polysaccharide containing mannose and galactofuranose as the main sugar components. The results of structural studies with methylation and NMR analyses on the native PHG and some of its specifically degraded products obtained on partial acid hydrolysis and acetolysis indicate that the polysaccharide moiety of the PHG has an (αl-6) linked mannan backbone with mainly (αl-2) linked side chains, each of which consists of 2 to 5 mannose units, and most of the mannosyl side chains bear β-galactofuranosyl residues linked to the 2 positions of the mannosyl nonreducing terminals. The galactofuranose residues are linked with each other by (β1-5) bonds.
amino acid, Galactomannan, proteoglycan, Neurospora crassa
Structure type: oligomer
Location inside paper: Fig.6, line 4
Compound class: mannan
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141795,IEDB_141830,IEDB_141834,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_164480,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: gel filtration, 13C NMR, 1H NMR, methylation, GC, amino acid analysis, enzymatic digestion, extraction, optical rotation measurement, acetylation, acetolysis, reduction, phenol-sulfuric acid assay, Lowry method
Enzymes that release or process the structure: exo-α-D-mannanase
Related record ID(s): 44129, 44130, 44131, 44132, 44134, 44135
NCBI Taxonomy refs (TaxIDs): 5141Reference(s) to other database(s): GTC:G95196FC
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error