Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
The structure was elucidated in this paperNCBI PubMed ID: 19279004Publication DOI: 10.1074/jbc.M807667200Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: jplatge
pasteur.fr
Institutions: Unité des Aspergillus, Institut Pasteur, Paris, France, Unité de Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur, Paris, France, Unité des Aspergillus, CNRS URA 2185, Institut Pasteur, Paris, France, Unité de Résonance Magnétique Nucléaire des Biomolécules, CNRS URA 2185, Institut Pasteur, Paris, France
Despite its essential role in the yeast cell wall, the exact composition of the β-(1,6)-glucan component is not well characterized. While solubilizing the cell wall alkali-insoluble fraction from a wild type strain of Saccharomyces cerevisiae using a recombinant β-(1,3)-glucanase followed by chromatographic characterization of the digest on an anion exchange column, we observed a soluble polymer that eluted at the end of the solvent gradient run. Further characterization indicated this soluble polymer to have a molecular mass of ~38 kDa and could be hydrolyzed only by β-(1,6)-glucanase. Gas chromatography, mass spectrometry and NMR(1H and 13C) analyses confirmed it to be a β-(1,6)-glucan polymer with, on average, branching at every fifth residue with one or two β-(1,3)-linked glucose units in the side chain. This polymer peak was significantly reduced in the corresponding digests from mutants of the kre genes (kre9 and kre5) that are known to play a crucial role in the β-(1,6)-glucan biosynthesis. In the current study, we have developed a biochemical assay wherein incubation of UDP-[14C]glucose with permeabilized S. cerevisiae yeasts resulted in the synthesis of a polymer chemically identical to the branched β-(1,6)-glucan isolated from the cell wall. Using this assay, parameters essential for β-(1,6)-glucan synthetic activity were defined.
mutants, cell wall, β-glucan, Saccharomyces cerevisiae, biochemical assay, carbon isotope
Structure type: oligomer
Location inside paper: Fig.3
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: gel filtration, 13C NMR, 1H NMR, methylation, GLC-MS, TLC, GC, Smith degradation, MALDI-TOF MS, radiolabeling, HPLC, enzymatic digestion, extraction, periodate oxidation, 15N NMR, reduction, column chromatography, cell growth, enzymatic assay, Bradford method, LPLC
Enzymes that release or process the structure: endo-β-(1,6)-glucanase
Related record ID(s): 44170, 44172, 44173, 44174, 44175, 44176, 44177, 44178
NCBI Taxonomy refs (TaxIDs): 1247190,
4932
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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