Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 22790168Publication DOI: 10.5650/jos.61.377Journal NLM ID: 101175339Publisher: Tokyo: Japan Oil Chemists Society
Correspondence: us.annapure
ictmumbai.edu.in
Institutions: Food Engineering and Technology Department, Institute of Chemical Technology, Mumbai, India
In present study, fermentative production of Sophorolipids (SLs) by Starmerella bombicola NRRL Y-17069 that have antibacterial activities was optimized. The media components like glucose, yeast extract and urea were critical components in the microbial growth and formation of SL moiety. The secondary carbon; oleic acid supports the formation of SLs. These components were optimized for maximum production of total SLs which have potential application. Optimization by one factor at-a-time method, glucose and yeast extract were found to be best sources for maximum production of SLs (g/L), 20.94 and 32.1 resp. Oleic acid (100 g/L) gave maximum production of 54.16 g/L with 54 % conversion of added oleic acid in fermentation media indicating fed batch operation is best for SLs production. Total sophorolipids formed were purified and 98.1% lactonic form was obtained by crystallization. Characterization of lactonic SLs by FT-IR, NMR and attached fatty acid moiety was confirmed by GCMS.
sophorolipid, Starmerella bombicola, oleic acid
Structure type: cyclic polymer repeating unit
Location inside paper: Fig.1, S1-S4, p.383, fig.9, p.384, fig.10
Compound class: sophorolipid
Contained glycoepitopes: IEDB_140628,IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, IR, GC-MS, biological assays, FTIR, extraction, cell growth, DNSA method
Related record ID(s): 44210
NCBI Taxonomy refs (TaxIDs): 75736
Show glycosyltransferases
NMR conditions: in CDCl3
1H NMR data: present in publication
|
13C NMR data: present in publication
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There is only one chemically distinct structure:
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 22790168Publication DOI: 10.5650/jos.61.377Journal NLM ID: 101175339Publisher: Tokyo: Japan Oil Chemists Society
Correspondence: us.annapure
ictmumbai.edu.in
Institutions: Food Engineering and Technology Department, Institute of Chemical Technology, Mumbai, India
In present study, fermentative production of Sophorolipids (SLs) by Starmerella bombicola NRRL Y-17069 that have antibacterial activities was optimized. The media components like glucose, yeast extract and urea were critical components in the microbial growth and formation of SL moiety. The secondary carbon; oleic acid supports the formation of SLs. These components were optimized for maximum production of total SLs which have potential application. Optimization by one factor at-a-time method, glucose and yeast extract were found to be best sources for maximum production of SLs (g/L), 20.94 and 32.1 resp. Oleic acid (100 g/L) gave maximum production of 54.16 g/L with 54 % conversion of added oleic acid in fermentation media indicating fed batch operation is best for SLs production. Total sophorolipids formed were purified and 98.1% lactonic form was obtained by crystallization. Characterization of lactonic SLs by FT-IR, NMR and attached fatty acid moiety was confirmed by GCMS.
sophorolipid, Starmerella bombicola, oleic acid
Structure type: oligomer
C
34H
58O
14Location inside paper: Fig.1, S5-S8
Trivial name: sophorolipid
Compound class: glycolipid, sophorolipid, glyccolipid
Contained glycoepitopes: IEDB_140628,IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, IR, GC-MS, biological assays, FTIR, extraction, cell growth, DNSA method
Related record ID(s): 44209, 49751, 49770, 49775, 49779, 49781, 49802, 49803, 49804, 49809, 49810
NCBI Taxonomy refs (TaxIDs): 75736
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Host organism: Tamarindus indica
Organ / tissue: fruit
NCBI PubMed ID: 17446065Publication DOI: 10.1016/j.biortech.2007.02.029Journal NLM ID: 9889523Publisher: Elsevier
Correspondence: Survase SA <shrikants.foodbio
udct.org>
Institutions: Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Mumbai, India
In the present work, four strains were screened for schizophyllan production, of which Schizophyllum commune NRCM was selected for further work. The fermentation was carried out for 168 h at 28±2 °C on an orbital shaker at 180 rpm. In the first step, one factor-at-a-time method was used to investigate the effect of media constituents such as carbon and nitrogen sources on schizophyllan production. Subsequently in the second step, concentration of the medium components was optimized using Response Surface Method (RSM). The yield increased from 3.25±0.72 g/l in the unoptimized media to 8.03±1.12 g/l in the medium optimized by RSM.
fermentation, schizophyllan, Schizophyllum commune, response surface method
Structure type: polymer chemical repeating unit
Location inside paper: abstract
Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, D-glucan, scleroglucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: acid hydrolysis, extraction, cell growth, spectrophotometry, centrifugation, phosphate measurement, DNSA method
Synthetic data: enzymatic in vivo
Related record ID(s): 42637, 42640, 43869, 43939, 44415, 44459, 45617, 45736, 45743, 45872, 49988, 50026, 50061, 50064, 50072, 50089, 116379
NCBI Taxonomy refs (TaxIDs): 5334Reference(s) to other database(s): GTC:G66305IS, CCSD:
49943, CBank-STR:12679, CA:9050-67-3
Show glycosyltransferases
There is only one chemically distinct structure:
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