Serotype-specific polysaccharide antigens from Actinobacillus actinomycetemcomitans ATCC 29523 (serotype a) and NCTC 9710 (serotype c) were extracted from whole cells by autoclaving and purified by ion-exchange chromatography and gel filtration. Analysis of component sugars by gas-liquid chromatography-mass spectrometry, high performance liquid chromatography, and NMR together with optical rotation data showed that the serotype a antigen was composed solely of 6-deoxy-D-talose, whereas the serotype c antigen consisted of 6-deoxy-L-talose. Structural analysis indicated that both of these antigens were composed of closely related repeating units, -3)-6-deoxy-α-D-Talp-(1-2)-6-deoxy-α-D-Talp-(1-(sero type a) and -3)-6-deoxy-α-L-Talp-(1-2)-6-deoxy-α-L-Talp-(1-(sero type c). 1H and 13C NMR analysis showed that both of these serotype antigens contained one acetyl group/2 sugar residues. These acetyl groups localized at the O-2 position of 3-linked 6-deoxy-D-talose (serotype a) or O-4 position of 3-linked 6-deoxy-L-talose residues (serotype c), respectively. These results coupled with our previous findings on the serotype b antigen (Amano, K., Nishihara, T., Shibuya, N., Noguchi, T., and Koga, T. (1989) Infect. Immun. 57, 2942-2946) showed that the serotype antigens from A. actinomycetemcomitans are a group of novel polysaccharides with structural features closely related biosynthetically.

6-deoxy-L-talose, Actinobacillus actinomycetemcomitans, polysaccharide antigen, 6-deoxy-D-talose, serotype-specific

• Compound ID: 1250
  

  -3)-a-L-6dTalp4Ac-(1-2)-a-L-6dTalp-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Trivial name: c-specific polysaccharide antigen
  Compound class: EPS, O-polysaccharide, O-antigen
  Reference(s) to other database(s): GTC:G66817OM, GlycomeDB:11555
  
   
  
  Actinobacillus actinomycetemcomitans c (NCTC 9710
  CSDB ID 28796 (all data & tools)
• Compound ID: 1287
  

  -3)-a-D-Fucp-(1-2)-a-L-Rhap-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Trivial name: b-specific polysaccharide antigen
  Compound class: O-polysaccharide, O-antigen, S-layer glycoprotein
  Reference(s) to other database(s): GTC:G48878CJ, GlycomeDB:3444
  
   
  
  Actinobacillus actinomycetemcomitans b (Y4)
  CSDB ID 28797 (all data & tools)
• Compound ID: 3046
  

  -3)-a-D-6dTalp2Ac-(1-2)-a-D-6dTalp-(1-

  Show graphically

  Show legend Show as text

  Structure type: polymer chemical repeating unit
  Trivial name: specific polysaccharide antigen (SPA), a-specific polysaccharide antigen
  Compound class: O-polysaccharide
  Reference(s) to other database(s): GTC:G35759NI, GlycomeDB:11556
  
   
  
  Actinobacillus actinomycetemcomitans a (ATCC 29523)
  CSDB ID 27380 (all data & tools)