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Structure type: polymer chemical repeating unit
Compound class: EPS
Contained glycoepitopes: IEDB_115136,IEDB_130701,IEDB_134624,IEDB_135614,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140630,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_156493,IEDB_190606,IEDB_241101,IEDB_423153,IEDB_742248,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 1679
  Tayama K, Minakami H, Fujiyama S, Masai H, Misaki A "Extracellular polysaccharides produced by acetic acid bacteria. Part III. Structure of an acidic polysaccharide elaborated by Acetobacter sp. NBI 1005" - Agricultural and Biological Chemistry 50 (1986) 1271-1278
  

  An extracellular acidic polysaccharide elaborated by Acetobacter sp. NBI 1005 was composed of D-glucose, D-galactose, D-mannose, and D-glucuronic acid (approximate molar ratio, 6:2:1:1). Methylation and fragmentation analysis by partial acid hydrolysis indicated that the polysaccharide has a branched structure containing a backbone chain of β-(l→4)-linked D-glucose residues, two out of every four glucose residues being substituted at the 0-3 positions to form two kinds of branches, one consisting of D-mannose and D-glucuronic acid residues and the other of (l→6)-linked D-galactose and D-glucose residues. © 1986, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.

  Publication DOI: 10.1271/bbb1961.50.1271
  Journal NLM ID: 0370452
  Publisher: Tokyo: Agricultural Chemical Society Of Japan
  Institutions: Nakano Biochemical Research Institute, Nakano Vinegar Co., Ltd., Handa, Aichi 475, Japan, Faculty of Science of Living, Osaka City University, Sugimoto-cho, Sumiyoshi, Osaka 558, Japan
  
   
  
  Acetobacter sp. NBI 1005
  CSDB ID 101228 (all data & tools)
• Article ID: 6303
  Rath T, Ruhmann B, Sieber V "Systematic optimization of exopolysaccharide production by Gluconacetobacter sp. and use of (crude) glycerol as carbon source" - Carbohydrate Polymers 276 (2022) 118769
  

  The usage of polysaccharides as biodegradable polymers is of growing interest in the context of a sustainable and ecofriendly economy. For this, the production of exopolysaccharides (EPS) by Gluconacetobacter sp. was investigated. Glycerol as carbon source revealed to be beneficial compared to glucose. In addition, pure glycerol could be substituted by a crude glycerol waste stream from biodiesel production. Systematic analysis of the peptone and phosphate concentrations in glycerol-based media indicated a strong effect of peptone. Optimized parameters resulted in a titer of 25.4 ± 2.4 g/L EPS with a productivity of 0.46 ± 0.04 g*(L*h)-1. With decreasing peptone, a variation in the monomer ratios was observed. An accompanying change in molecular size distribution indicated the production of two different polysaccharides. Intensified analysis revealed the main polysaccharide to be composed of glucose (Glc), galactose (Gal), mannose (Man) and glucuronic acid (GlcA), and the minor polysaccharide of Gal, Man, ribose (Rib).

  exopolysaccharide, optimization

  NCBI PubMed ID: 34823788
  Publication DOI: 10.1016/j.carbpol.2021.118769
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: V. Sieber
  Institutions: Chair of Chemistry of Biogenic Resources, Technical University of Munich, Campus for Biotechnology and Sustainability, 94315 Straubing, Germany, Fraunhofer IGB, Branch BioCat, 94315 Straubing, Germany, Catalysis Research Center, Technical University of Munich, 85748 Garching, Germany, The University of Queensland, School of Chemistry and Molecular Biosciences, 68 Cooper Road, St. Lucia 4072, Australia
  Methods: sugar analysis, GPC, fermentation, media composition, preculture preparation, UHPLC-UV-ESI-MS/MS, crude glycerol analysis
  
   
  
  Acetobacter pasteurianus subsp. estunensis, Gluconacetobacter capsulatus IAM 1813, Acetobacter sp. NBI 1005
  CSDB ID 8537 (all data & tools)