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Bi H, Gao T, Liu D, Tai G, Wei M, Zhou Y
Structures of (1→6)-β-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
Carbohydrate Polymers 93(2) (2013)
547-552
|
b-D-Glcp-(1-3)-+
|
-6)-b-D-Glcp-(1-6)-{{{-b-D-Glcp-(1-6)-}}}/n=83/-b-D-Glcp-(1- |
Show graphically |
Bulgaria inquinans
(NCBI TaxID 73172,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 23499095Publication DOI: 10.1016/j.carbpol.2012.12.036Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhou Y <zhouyf383
yahoo.cn>; Zhou Y <zhouyf383
nenu.edu.cn>
Institutions: School of Life Sciences, Northeast Normal University, Changchun , China, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
In previous study, an unbranched (1→6)-β-D-glucan with Mw 2.6kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1→6)-β-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5kDa (BIK2), 288.9kDa (BIK10) and 175.5kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four β-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1→6)-β-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1→6)-β-D-glucan, and the effect of (1→6)-β-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1→6)-β-D-glucan, and indicate (1→6)-β-D-glucans from B. inquinans (Fries) are potential immunostimulating agents.
polysaccharide, Bulgaria inquinans (Fries), lymphocyte proliferation, (1→6)-β-D-glucan
Structure type: polymer chemical repeating unit ; n=3, 37500
Location inside paper: Fig. 3, Fig. 2, table 3, BIK2
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, gel filtration, GC, Smith degradation, HPLC, UV, optical rotation measurement, methylation analysis, phenol-sulfuric acid assay, lymphocyte proliferation assay, monosaccharide analysis, dyalysis
Biological activity: comprative analysis of BIK2, BIK10, BIK30 и BIWP2 revealed molecular weight and the branching ratio influence on immonological activities; the (1→6)-β-D-glucan with higher of branching ration and molecular weight shows higher effect on lymphocytes proliferation; The molecular weight and the branching ratio influence the binding capacity between (1→6)-β-D-glucans and β-glucan receptor, and molecular weight is more effective than branching ratio
Related record ID(s): 44874, 44875, 44876
NCBI Taxonomy refs (TaxIDs): 73172Reference(s) to other database(s): GTC:G01635LQ
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 293(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 bDGlcp 103.4 73.6 76.7 70.0 75.7 68.6
6,6 bDGlcp 103.4 73.6 76.7 70.0 75.7 68.6
3 bDGlcp 103.2 73.6 76.7 70.0 75.7 61.1
bDGlcp 103.4 73.6 86.2 70.0 76.7 68.6
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | bDGlcp | 103.4 | 73.6 | 76.7 | 70.0 | 75.7 | 68.6 |
| 6,6 | bDGlcp | 103.4 | 73.6 | 76.7 | 70.0 | 75.7 | 68.6 |
| 3 | bDGlcp | 103.2 | 73.6 | 76.7 | 70.0 | 75.7 | 61.1 |
| | bDGlcp | 103.4 | 73.6 | 86.2 | 70.0 | 76.7 | 68.6 |
|
There is only one chemically distinct structure:
Expand this record
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Bi H, Gao T, Liu D, Tai G, Wei M, Zhou Y
Structures of (1→6)-β-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
Carbohydrate Polymers 93(2) (2013)
547-552
|
b-D-Glcp-(1-3)-+
|
-6)-b-D-Glcp-(1-6)-{{{-b-D-Glcp-(1-6)-}}}/n=3/-b-D-Glcp-(1- |
Show graphically |
Bulgaria inquinans
(NCBI TaxID 73172,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 23499095Publication DOI: 10.1016/j.carbpol.2012.12.036Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhou Y <zhouyf383
yahoo.cn>; Zhou Y <zhouyf383
nenu.edu.cn>
Institutions: School of Life Sciences, Northeast Normal University, Changchun , China, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
In previous study, an unbranched (1→6)-β-D-glucan with Mw 2.6kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1→6)-β-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5kDa (BIK2), 288.9kDa (BIK10) and 175.5kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four β-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1→6)-β-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1→6)-β-D-glucan, and the effect of (1→6)-β-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1→6)-β-D-glucan, and indicate (1→6)-β-D-glucans from B. inquinans (Fries) are potential immunostimulating agents.
polysaccharide, Bulgaria inquinans (Fries), lymphocyte proliferation, (1→6)-β-D-glucan
Structure type: polymer chemical repeating unit ; n=297, 299800
Location inside paper: Fig. 3, Fig. 2, table 3, BIK10
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, gel filtration, GC, Smith degradation, HPLC, UV, optical rotation measurement, methylation analysis, phenol-sulfuric acid assay, lymphocyte proliferation assay, monosaccharide analysis, dyalysis
Biological activity: comprative analysis of BIK2, BIK10, BIK30 и BIWP2 revealed molecular weight and the branching ratio influence on immonological activities; the (1→6)-β-D-glucan with higher of branching ration and molecular weight shows higher effect on lymphocytes proliferation; The molecular weight and the branching ratio influence the binding capacity between (1→6)-β-D-glucans and β-glucan receptor, and molecular weight is more effective than branching ratio
Related record ID(s): 44873, 44875, 44876
NCBI Taxonomy refs (TaxIDs): 73172
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 293(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 bDGlcp 103.4 73.6 76.6 70.1 75.7 68.7
6,6 bDGlcp 103.4 73.6 76.6 70.1 75.7 68.7
3 bDGlcp 103.3 73.6 76.6 70.1 75.7 61.2
bDGlcp 103.4 73.6 86.2 70.1 76.6 68.7
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | bDGlcp | 103.4 | 73.6 | 76.6 | 70.1 | 75.7 | 68.7 |
| 6,6 | bDGlcp | 103.4 | 73.6 | 76.6 | 70.1 | 75.7 | 68.7 |
| 3 | bDGlcp | 103.3 | 73.6 | 76.6 | 70.1 | 75.7 | 61.2 |
| | bDGlcp | 103.4 | 73.6 | 86.2 | 70.1 | 76.6 | 68.7 |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Bi H, Gao T, Liu D, Tai G, Wei M, Zhou Y
Structures of (1→6)-β-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
Carbohydrate Polymers 93(2) (2013)
547-552
|
b-D-Glcp-(1-3)-+
|
-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-b-D-Glcp-(1- |
Show graphically |
Bulgaria inquinans
(NCBI TaxID 73172,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 23499095Publication DOI: 10.1016/j.carbpol.2012.12.036Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhou Y <zhouyf383
yahoo.cn>; Zhou Y <zhouyf383
nenu.edu.cn>
Institutions: School of Life Sciences, Northeast Normal University, Changchun , China, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
In previous study, an unbranched (1→6)-β-D-glucan with Mw 2.6kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1→6)-β-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5kDa (BIK2), 288.9kDa (BIK10) and 175.5kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four β-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1→6)-β-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1→6)-β-D-glucan, and the effect of (1→6)-β-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1→6)-β-D-glucan, and indicate (1→6)-β-D-glucans from B. inquinans (Fries) are potential immunostimulating agents.
polysaccharide, Bulgaria inquinans (Fries), lymphocyte proliferation, (1→6)-β-D-glucan
Structure type: polymer chemical repeating unit ; n=271, 175500
Location inside paper: Fig. 3, Fig. 2, table 3, BIK30
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, gel filtration, GC, Smith degradation, HPLC, UV, optical rotation measurement, methylation analysis, phenol-sulfuric acid assay, lymphocyte proliferation assay, monosaccharide analysis, dyalysis
Biological activity: comprative analysis of BIK2, BIK10, BIK30 и BIWP2 revealed molecular weight and the branching ratio influence on immonological activities; the (1→6)-β-D-glucan with higher of branching ration and molecular weight shows higher effect on lymphocytes proliferation; The molecular weight and the branching ratio influence the binding capacity between (1→6)-β-D-glucans and β-glucan receptor, and molecular weight is more effective than branching ratio
Comments, role: polymerization frame was shifted due to conformity with other records
Related record ID(s): 44873, 44874, 44876
NCBI Taxonomy refs (TaxIDs): 73172Reference(s) to other database(s): GTC:G37413PU
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 293(C) K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 bDGlcp 103.4 73.7 76.7 70.0 75.7 68.6
6,3 bDGlcp 103.2 73.7 76.7 70.0 75.7 61.1
6 bDGlcp 103.4 73.7 86.3 70.0 76.7 68.6
bDGlcp 103.4 73.7 76.7 70.0 75.7 68.6
1H NMR data:
missing...
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | bDGlcp | 103.4 | 73.7 | 76.7 | 70.0 | 75.7 | 68.6 |
| 6,3 | bDGlcp | 103.2 | 73.7 | 76.7 | 70.0 | 75.7 | 61.1 |
| 6 | bDGlcp | 103.4 | 73.7 | 86.3 | 70.0 | 76.7 | 68.6 |
| | bDGlcp | 103.4 | 73.7 | 76.7 | 70.0 | 75.7 | 68.6 |
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Bi H, Gao T, Liu D, Tai G, Wei M, Zhou Y
Structures of (1→6)-β-D-glucans from Bulgaria inquinans (Fries) and their immunological activities
Carbohydrate Polymers 93(2) (2013)
547-552
Bulgaria inquinans
(NCBI TaxID 73172,
species name lookup)
Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 23499095Publication DOI: 10.1016/j.carbpol.2012.12.036Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: Zhou Y <zhouyf383
yahoo.cn>; Zhou Y <zhouyf383
nenu.edu.cn>
Institutions: School of Life Sciences, Northeast Normal University, Changchun , China, Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, China
In previous study, an unbranched (1→6)-β-D-glucan with Mw 2.6kDa was isolated from fruit bodies of Bulgaria inquinans (Fries). In present paper, three branched (1→6)-β-D-glucans were obtained from the water-extracted residues by a sequential KOH-extraction, namely BIK2, BIK10 and BIK30. Their molecular weights were determined to be 37.5kDa (BIK2), 288.9kDa (BIK10) and 175.5kDa (BIK30). Structural analysis indicated that their backbones were substituted by single glucosyls at C-3 positions, the branching ratios were 0.01 (BIK2), 0.17 (BIK10), 0.25 (BIK30). Immunological tests showed that all the four β-D-glucans could significantly increase the ConA or LPS-induced lymphocytes proliferation in vivo. Moreover, branched (1→6)-β-D-glucans have more significantly lymphocytes proliferation activities than unbranched (1→6)-β-D-glucan, and the effect of (1→6)-β-D-glucans on lymphocytes proliferation increases along with molecular weights. The present results well enrich the structure-activity relationships of (1→6)-β-D-glucan, and indicate (1→6)-β-D-glucans from B. inquinans (Fries) are potential immunostimulating agents.
polysaccharide, Bulgaria inquinans (Fries), lymphocyte proliferation, (1→6)-β-D-glucan
Structure type: homopolymer ; n=9, 2600
Location inside paper: conclusion, BIWP2
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, periodate oxidation, gel filtration, GC, Smith degradation, HPLC, UV, optical rotation measurement, methylation analysis, phenol-sulfuric acid assay, lymphocyte proliferation assay, monosaccharide analysis, dyalysis
Biological activity: comprative analysis of BIK2, BIK10, BIK30 и BIWP2 revealed molecular weight and the branching ratio influence on immonological activities; the (1→6)-β-D-glucan with higher of branching ration and molecular weight shows higher effect on lymphocytes proliferation; The molecular weight and the branching ratio influence the binding capacity between (1→6)-β-D-glucans and β-glucan receptor, and molecular weight is more effective than branching ratio
Related record ID(s): 44873, 44874, 44875
NCBI Taxonomy refs (TaxIDs): 73172Reference(s) to other database(s): GTC:G26777BZ
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
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Total list of record IDs on all result pages of the current query:
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