Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 23178399Publication DOI: 10.1016/j.ijbiomac.2012.11.014Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Islam SS <sirajul_1999
yahoo.com>
Institutions: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India, Department of Biotechnology, Indian Institute of Technology (IIT) Kharagpur, Kharagpur, West Bengal, India, Division of Plant Biology, Bose Institute, Centenary Building, P-1/12, C.I.T. Scheme VII M, Kolkata, West Bengal, India
A water-soluble glucan (PS-I) was isolated from the aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-I was carried out using total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Methylation analysis revealed that PS-I was composed of (1→3, 6), (1→3), (1→6)-linked and terminal β-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. The repeating unit of the glucan consists of a backbone chain of two (1→6)-β-D-glucopyranosyl residues, one of which is branched at O-3 position with (1→3)-β-D-glucopyranosyl and terminated with a β-D-glucopyranosyl residue. Study of immunological activity revealed that PS-I stimulates the splenocytes, thymocytes and macrophages.
NMR, polysaccharide, macrophages, splenocytes and thymocytes
Structure type: polymer chemical repeating unit
Location inside paper: Table 1, p. 131, column 2, first figure, PS-I
Compound class: glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, acid hydrolysis, periodate oxidation, ROESY, TOCSY, reduction with NaBH4, DQF-COSY, HMBC, NOESY, HSQC, nitric oxide assay, GC–MS, splenocyte proliferation assay, thymocyte proliferation assay, GLS
Biological activity: This glucan activates splenocytes and thymocytes and interacts with cell surface receptor molecules. Viability of splenocytes and thymocytes was over 90%. Splenocyte and thymocyte proliferation indices were found to be maximum at 50 μg/mL.
NCBI Taxonomy refs (TaxIDs): 188765,
38802Reference(s) to other database(s): GTC:G02185KR
Show glycosyltransferases
NMR conditions: in D2O at 303 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6 bDGlcp 102.9 73.0 75.5 69.6 74.9 68.8
3,3 bDGlcp 102.7 73.0 75.6 69.6 75.9 60.7
3 bDGlcp 103.0 72.9 84.2 69.5 75.9 60.7
bDGlcp 103.0 72.9 84.2 69.5 74.9 68.6
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6 bDGlcp 4.52 3.33 3.50 3.46 3.65 3.88-4.22
3,3 bDGlcp 4.52 3.35 3.50 3.41 3.48 3.72-3.91
3 bDGlcp 4.54 3.52 3.73 3.48 3.48 3.73-3.92
bDGlcp 4.54 3.52 3.74 3.48 3.64 3.86-4.21
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6 bDGlcp 102.9/4.52 73.0/3.33 75.5/3.50 69.6/3.46 74.9/3.65 68.8/3.88-4.22
3,3 bDGlcp 102.7/4.52 73.0/3.35 75.6/3.50 69.6/3.41 75.9/3.48 60.7/3.72-3.91
3 bDGlcp 103.0/4.54 72.9/3.52 84.2/3.73 69.5/3.48 75.9/3.48 60.7/3.73-3.92
bDGlcp 103.0/4.54 72.9/3.52 84.2/3.74 69.5/3.48 74.9/3.64 68.6/3.86-4.21
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6 | bDGlcp | 4.52 | 3.33 | 3.50 | 3.46 | 3.65 | 3.88
4.22 |
| 3,3 | bDGlcp | 4.52 | 3.35 | 3.50 | 3.41 | 3.48 | 3.72
3.91 |
| 3 | bDGlcp | 4.54 | 3.52 | 3.73 | 3.48 | 3.48 | 3.73
3.92 |
| | bDGlcp | 4.54 | 3.52 | 3.74 | 3.48 | 3.64 | 3.86
4.21 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6 | bDGlcp | 102.9 | 73.0 | 75.5 | 69.6 | 74.9 | 68.8 |
| 3,3 | bDGlcp | 102.7 | 73.0 | 75.6 | 69.6 | 75.9 | 60.7 |
| 3 | bDGlcp | 103.0 | 72.9 | 84.2 | 69.5 | 75.9 | 60.7 |
| | bDGlcp | 103.0 | 72.9 | 84.2 | 69.5 | 74.9 | 68.6 |
|
There is only one chemically distinct structure:
Found 
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(later renamed to: Pleurotus sp. 'Florida' x Lentinus squarrosulus)