Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
NCBI PubMed ID: 7911123Publication DOI: 10.1111/j.1349-7006.1994.tb02374.xJournal NLM ID: 8509412Publisher: Tokyo: Japanese Cancer Association
Institutions: Basic Research Laboratories, Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan, Institute for Immunology, Faculty of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, Japan
Lentinan, an antitumor polysaccharide used clinically in Japan, requires the intact T cell compartment to manifest its antitumor effects. The aim of the current study was to clarify the mechanisms playing crucial roles in the T cell requirement in the expression of antitumor effects of lentinan. Lentinan treatment of BDF1 mice transplanted intradermally with FBL-3 induced complete tumor regression and a marked increase in survival time. The antitumor action of lentinan was abolished in mice treated simultaneously with antibodies to CD4 and CD8 antigens, whereas antibody to CD4, CD8 or NK1.1 alone was ineffective. The natural killer, cytotoxic T lymphocyte, and helper T cell activities were already augmented in this FBL-3/BDF1 system and thus further augmentation of these activities by lentinan was not observed. These activities did not correlate with the antitumor activity of lentinan, as was confirmed in lymphocyte subset depletion experiments. On the contrary, the delayed-type hypersensitivity (DTH) response against tumor-associated antigens was triggered by lentinan and was abrogated only in mice treated simultaneously with antibodies to CD4 and CD8 antigens. Furthermore, a non-cytolytic tumor-associated antigen-specific CD4+ T cell clone able to induce the DTH response in concert with lentinan reconstituted the antitumor effects in B6 nude mice when administered with lentinan. These results suggest that, in addition to the augmentation of immune effector cell activity against tumors, infiltration of these cells into the tumor burden initiated by the DTH responses at tumor sites may be involved in eradication of tumors by lentinan.
lentinan, antitumor effect, DTH, CD4 T cell clone, effector infiltration
Structure type: structural motif or average structure ; 600000
Location inside paper: lentinan, p.410
Trivial name: lentinan
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: biological assay, antitumor activity assay, interleukin-2 assay
Biological activity: 87.5% of mice showed complete tumor regression after lentinan abdominally administration
Related record ID(s): 45376, 45377, 45378, 45379, 45380, 45383, 45384, 45385, 45387, 45388, 45389, 45390, 45391, 45392, 45394, 45395, 45396, 45397, 45398, 45399, 45405, 45408, 48347, 48358, 48474, 48490, 49209
NCBI Taxonomy refs (TaxIDs): 5353Reference(s) to other database(s): GTC:G14698DR
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There is only one chemically distinct structure:
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(later renamed to: Lentinula edodes)