Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 15885873Publication DOI: 10.1016/j.biochi.2005.03.004Journal NLM ID: 1264604Publisher: Paris: Editions Scientifiques Elsevier
Correspondence: Tan RX <rxtan
nju.edu.cn>; Gao XD <xiangdong_gao
hotmail.com>
Institutions: Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China
YCP, a mitogenic polysaccharide with its molecular weight (MW) of 2.4 x 10^3 kDa, was isolated from the mycelium of the marine filamentous fungus Phoma herbarum YS4108 by a combination of ion-exchange chromatography on DEAE-32 and gel permeation over Sephacryl S-400. The detailed compositional, spectroscopic and methylation analyses of the polysaccharide demonstrated that its backbone possessed most likely a linear α-(1-4) bonded glucopyranoside main chain co-bearing through side α-(1-6)-linkage. The α-(1-4) bondage of the glucopyranoside building blocks in YCP was confirmed by the observation that it could be hydrolyzed by the α-amylase produced by Bacillus licheniformis. A reliable concentration monitoring experimentation highlighted that the reducing sugars released continuously from YCP during its incubation with the enzyme, and the MW of the main resulting fragment weighed 0.8 x 10^4 Da with approximately 10% of YCP converted to maltose, maltotriose and glucose after a 120-min enzymatic degradation. Finally, YCP was found to be able to increase phagocytic activity of mice in vitro and in vivo, indicating that it may be looked up as a potent immunomodulator that could activate macrophages.
Phoma herbarum, a-amylase, YCP, enzymatic modification, mitogenic activity
Structure type: homopolymer
Location inside paper: YCP, p.750, column 2, fig.4
Trivial name: methyl glucose lipopolysaccharide, glucan, maltosaccharide, α-1,4-D-glucan, amylose, α-glucan, glycogen backbone, α-(1,4)-glucan, starch, α-(1-4)-glucan, starch, glycogen
Compound class: EPS, O-polysaccharide, CPS, cell wall polysaccharide, glucan, polysaccharide, methyl glucose lipopolysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, TLC, FTIR, HPSEC, UV, ion-exchange chromatography, extraction, optical rotation measurement, methylation analysis, column chromatography, enzymatic assay, precipitation, Sevag method, phagocytosis assay, mitogenic assay
Biological activity: absorbance (neutral red) of YCP-treating groups at 100 and 200 µg/ml reached 0.16 and 0.22; caused substantial increments in phagocytic activity of mice at concentrations of 3 and 9 mg/kg by 2.5- and 3.0-fold
Enzymes that release or process the structure: α-amylase
Comments, role: backbone chain (90.4 mol%); total mass 2400000
Related record ID(s): 47107, 50866
NCBI Taxonomy refs (TaxIDs): 73001Reference(s) to other database(s): GTC:G05740LL, GlycomeDB:
12100, CCSD:
4943, CBank-STR:819
Show glycosyltransferases
NMR conditions: in D2O at 363 K
1H NMR data: present in publication
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13C NMR data: present in publication
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There is only one chemically distinct structure:
Found 
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