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1. (Article ID: 4572)
 
Marchetti R, Canales A, Lanzetta R, Nilsson I, Vogel C, Reed DE, Aucoin DP, Jiménez-Barbero J, Molinaro A, Silipo A
Unraveling the Interaction between the LPS O-Antigen of Burkholderia anthina and the 5D8 Monoclonal Antibody by Using a Multidisciplinary Chemical Approach, with Synthesis, NMR, and Molecular Modeling Methods
Chembiochem: a European Journal of Chemical Biology 14(12) (2013) 1485-1493
 

The interaction between the O-chain from the lipopolysaccharide from Burkholderia anthina and a lipopolysaccharide-specific monoclonal antibody (5D8) has been studied at high resolution by NMR spectroscopy. In particular, the 5D8-bound epitope of the saccharide entity has been unraveled by a combination of saturation transfer difference (STD) and transferred NOESY (tr-NOESY) experiments performed on the 5D8/polysaccharide complex. To dissect the fine details of the molecular recognition events, further experiments with simpler carbohydrate ligands were carried out. Thus, experiments were also performed with ad hoc synthesized trisaccharide and hexasaccharide O-antigen repeating units. By using this multidisciplinary approach (chemical synthesis, NMR spectroscopy and molecular dynamics simulation), determination of the binding epitope and the contribution to the binding of the sugar units composing the O-chain have been determined.

NMR spectroscopy, molecular modeling, Burkholderia anthina, LPS–antibody interaction

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2. (Article ID: 4778)
 
Nilsson I, Michalik D, Silipo A, Molinaro A, Vogel C
Efficient synthesis of O-antigen fragments expressed by Burkholderia anthina by modular synthesis approach
Carbohydrate Research 404 (2015) 98-107
 

To facilitate mapping of the interaction region of the O-chain of the lipopolysaccharide from Burkholderia anthina and of a lipopolysaccharide-specific monoclonal antibody, trisaccharide propyl α-L-rhamnopyranosyl-(1→2)-α-D-galactopyranosyl-(1→3)-α-L-rhamnopy ranoside (27) and hexasaccharide propyl α-L-rhamnopyranosyl-(1→2)-α-D-galactopyranosyl-(1→3)-α-L-rhamnopy ranosyl-(1→2)-α-L-rhamnopyranosyl-(1→2)-α-D-galactopyranosyl-(1→3)-α-L-rhamnopyranoside (33) were synthesized. These oligosaccharides represent the repeating monomer and dimer of the O-antigen, respectively.

O-antigen, Oligosaccharides, L-rhamnose, glycosylation, D-galactose, Burkholderia anthina

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