Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
Organ / tissue: cell wall
Publication DOI: 10.1016/S0144-8617(03)00162-0Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: freimund
tech.chem.ethz.ch
Institutions: Institute for Chemical and Bioengineering, ETH Hönggerberg, Zürich, Switzerland, ABAC R&D AG, Schlieren, Switzerland
1,3-β-D-glucan is a widespread polysaccharide in microorganisms, mushrooms and plants. Numerous benefits for the health of humans and animals have been described for more than 50 years. One readily available source for 1,3-β-D-glucan is the cell wall of the common baker’s or brewer’s yeast Saccharomyces cerevisiae. Conventional isolation processes involving treatments with hot alkali and acids, however, cause a certain degradation of the polymeric chains resulting not only in reduced yields but also a possible weakening of the beneficial effects. We have developed a new non-degrading process for the isolation of glucan from yeast cell walls comprising gentle extraction steps and enzymatic treatments without any extreme pH values. Depending on the quality of the utilized yeast cell walls, 1,3-β-D-glucan is obtained in a purity of up to 92% and a yield of 87%. During this process, the valuable byproduct mannoprotein - also beneficial for health - is easily obtained.
1, Saccharomyces cerevisiae, 3-β-D-glucan, mannoprotein, yeast cell walls
Structure type: homopolymer
Location inside paper: Fig. 1, Fig. 4, Fig. 5
Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, callose, laminarin, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP)
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, cell wall glucan, polysaccharide, glycoside, β-glucan, β-1, 3-glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, acid hydrolysis, GC, alkaline hydrolysis, enzymatic digestion, elemental analysis, precipitation, derivatization, centrifugation, Lowry method
Related record ID(s): 42641
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:
157, CCSD:
50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
Show glycosyltransferases
NMR conditions: in DMSO-d6 at 393 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
bDGlcp 102.49 72.46 85.76 68.18 76.02 60.67
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
bDGlcp 4.553 3.349 3.514 3.31 3.31 3.518-3.746
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
bDGlcp 102.49/4.553 72.46/3.349 85.76/3.514 68.18/3.31 76.02/3.31 60.67/3.518-3.746
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | bDGlcp | 4.553 | 3.349 | 3.514 | 3.31 | 3.31 | 3.518
3.746 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | bDGlcp | 102.49 | 72.46 | 85.76 | 68.18 | 76.02 | 60.67 |
|
There is only one chemically distinct structure:
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