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1. (Article ID: 4617)
 
Sigida EN, Fedonenko YP, Shashkov AS, Zdorovenko EL, Konnova SA, Ignatov VV, Knirel YA
Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7
Carbohydrate Research 380 (2013) 76-80
 

Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (~40%) 2-O-methylation of l-rhamnose.

Lipopolysaccharide, Azospirillum brasilense, bacterial polysaccharide structure

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