Production of Candida bombicola sophorolipid (SL) was studied using a mixture of motor oil waste and sunflower oil cake as a substrate. In this work, submerged and solid state fermentation (SSF) techniques were used. The highest yield was achieved with SSF giving two fractions methanol fraction (F I) of 4.75 g% and ethyl acetate fraction (F II) of 41.77g%. To optimize the production, time course was studied. The minimum surface tension and critical micelle concentrations (CMC) were obtained at the 12th day of fermentation for both fractions, giving 36.2 mN/m and 45.2 mN/m at CMC of 68.5 mgl-1 and 391.4 mgl-1, respectively. Emulsification activity with different hydrocarbons and the stability against pH, temperature, and salinity studies revealed that both fractions showed high tolerance for these environmental factors, suggesting their potential applications in extreme industrial and environmental conditions. The efficiency of the produced SLs for microbiologically enhanced oil recovery (MEOR) was 71.43% and 72.73%, respectively. FTIR and NMR spectroscopy analyses confirmed the structure of the produced compounds.

characterization, sophorolipids, Candida bombicola, motor oil waste, sunflower oil cake

13C NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

R1 = LIP
[1*]O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O

425.363 g/mol (C₁₆H₂₅RO₁₃, R = residue superclass(es), not counted in mol weight)