CSDB: Search results

Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)

The structure was elucidated in this paper
Publication DOI: 10.1177/0883911518793914
Journal NLM ID: 8710961
Publisher: London: SAGE Publications
Correspondence: Radhouani H <hajer.radhouani

i3bs.uminho.pt>
Institutions: 3B’s Research Group, I3Bs—Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal, ICVS/3B’s-PT Government Associate Laboratory, Braga, Portugal, The Discoveries Centre for Regenerative and Precision Medicine, University of Minho, Guimarães, Portugal

Kefiran, an exopolysaccharide produced by lactic acid bacteria, has received a great interest due to a variety of health claims. In this study, we aim to investigate the physicochemical and biological properties of Kefiran polysaccharide extracted from Portuguese kefir grains. The kefir growth rate was about 56% (w/w) at room temperature and the kefir pH after 24 h was about 4.6. The obtained yield of Kefiran polysaccharide extracted from the kefir grains was about 4.26% (w/w). The Kefiran structural features were showed in the 1H nuclear magnetic resonance spectrum. The bands observed in the infrared spectrum confirmed that the Kefiran had a β-configuration; and the X-ray photoelectron spectroscopy analysis confirmed the structure and composition of Kefiran and revealed a C/O atomic ratio of 1.46. Moreover, Kefiran showed an average molecular weight (Mw) of 534 kDa and a number-average molecular weight (Mn) of 357 kDa. Regarding the rheological data obtained, Kefiran showed an interesting adhesive performance accompanied by a pseudoplastic behavior, and the extrusion force of Kefiran was 1 N. Furthermore, Kefiran exhibited a higher resistance to hyaluronidase degradation than hyaluronic acid. Finally, Kefiran showed a lack of cytotoxic response through its ability to support metabolic activity and proliferation of L929 cells, and had no effect on these cells’ morphology. Our research suggested that Kefiran polymer has attractive and interesting properties for a wide range of biomedical applications, such as tissue engineering and regenerative medicine.

cytotoxicity, exopolysaccharide kefiran, drug delivery system, enzymatic resistance, physicochemical characterization, Portuguese kefir grains

Structure type: structural motif or average structure ; 357000-534000
Location inside paper: fig.1
Trivial name: kefiran
Compound class: EPS
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_153218,IEDB_190606,IEDB_221845,IEDB_241101,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88

Methods: 1H NMR, X-ray, FTIR, differential scanning calorimetry (DSC), extraction, optical rotation measurement, dialysis, enzymatic assay, cytotoxicity assay, HPGPC, simultaneous thermal analysis, rheological study, oscillatory experiments
Biological activity: showed a lack of cytotoxic response through its ability to support metabolic activity and proliferation of L929 cells
Comments, role: BA is shown for kefiran from Portuguese kefir grains; -6)bDGlcp(1-?)[bDGlcp(1-?)]bDGalp means that bDGalp is substituted at 2 or 6 positions

NCBI Taxonomy refs (TaxIDs): 1578, 267818
Reference(s) to other database(s): GTC:G91956TA
Show glycosyltransferases

NMR conditions: in D2O at 333(H) K [as TSV]

13C NMR data:
missing...

¹H NMR data: present in publication

There are 12 chemically distinct structures. Please, select:

*OC[C@H]1O[C@@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](O[C@H]3[C@@H](CO)O[C@@H](O[C@H]4[C@@H](O)[C@@H](CO)O[C@@H](O[C@@H]5[C@@H](CO)O[C@@H](*)[C@H](O)[C@H]5O)[C@@H]4O)[C@H](O)[C@H]3O)[C@@H]2O[C@@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

SVG MW SMILES 3D mol Ionize