Taxonomic group: bacteria, fungi / Proteobacteria, Ascomycota
(Phylum: Proteobacteria, Ascomycota)
The structure was elucidated in this paperNCBI PubMed ID: 20096763Publication DOI: 10.1016/j.vaccine.2010.01.012Journal NLM ID: 8406899Publisher: Elsevier
Correspondence: antonio.cassone
iss.it
Institutions: Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy, Research Center, Novartis Vaccines and Diagnostics, Siena, Italy
A laminarin-diphtheria toxoid (CRM197) conjugate vaccine conferred protection against fungal infections in mice. We have now generated novel β-glucan-CRM197 vaccines, with either natural (Curd-CRM197) or synthetic linear (15mer-CRM197), or β-(1,6)-branched (17mer-CRM197) β-(1,3)-oligosaccharides, formulated with the human-acceptable adjuvant MF59. Curd-CRM197 and 15mer-CRM197 conjugates, which induced high titers of anti-β-(1,3)-glucan IgG, but no antibodies against β-(1,6)-glucan, conferred protection to mice lethally challenged with C. albicans. In contrast, the 17mer-CRM197 conjugate, which induced anti-β-(1,6)-glucan antibodies in addition to the anti-β-(1,3)-glucan IgG, was non-protective. These data provide some insights on β-glucan epitope(s) mediating antifungal protection and open the way to develop a synthetic oligosaccharide vaccine against fungal diseases.
adjuvant, β-glucan, antifungal vaccine, antifungal antibody
Structure type: homopolymer
Location inside paper: fig.1, p.2616 (2.1 Reagents, GG-Zym)
Aglycon: diphtheria toxoid (CRM197)
Trivial name: curdlan, Curd-CRM197, 15mer-CRM197, zymosan
Compound class: glucan
Contained glycoepitopes: IEDB_1397514,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 1H NMR, SDS-PAGE, ELISA, chemical synthesis, MS, HPSEC, surface plasmon resonance (SPR), biological assay
Biological activity: Curd-CRM197 bound by the antibody with KD 9.22 nM; 15mer-CRM197 (n=15) bound by the antibody with KD 1.62 nM; Immunization of mice with the Curd-CRM197 or the synthetic 15mer-CRM197 conjugates raised high titers of specific anti-β-glucan IgG antibodies that recognized Lam and Candida GG-Zym, but no or very low levels of antibodies recognizing pustulan
Synthetic data: chemical
Related record ID(s): 47151, 47153, 50617, 50648, 50654, 50665, 50671, 50861
NCBI Taxonomy refs (TaxIDs): 511,
5476Reference(s) to other database(s): GTC:G51056AN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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