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1. (Article ID: 4724)
 
Sigida EN, Fedorova EE, Zdorovenko EL, Burygin GL, Konnova SA, Ignatov VV
Characterization of the lipopolysaccharides of serogroup II Azospirillum strains
Mikrobiologiia = Microbiology [Russian] 83(4) (2014) 326-334
 

Lipopolysaccharides of six Azospirillum brasilense strains (SR50, SR80, SR88, SR109, SR111, SR115) and A. lipoferum SR 42 isolated from the rhizosphere of cereal plants of Saratov oblast, Russia and referred to serogroup II by serological analysis were studied. In the lipid A fatty acid composition, the lipopolysaccharides under study were similar to those of other Azospirillum strains and were characterized by a predominance of 3-hydroxytetradecanoic, 3-hydroxyhexadecanoic, and octadecenoic acids. Monosaccharide analysis of the O-specific polysaccharides (including determination of the absolute configurations, methylation analysis, and one- and two-dimensional NMR spectroscopy) revealed the presence of two types of repeating units in varying ratios. A high degree of serological similarity between the strains under study was shown to result from the presence of repeating units with identical structure of their O antigens.

lipopolysaccharides, Azospirillum, structure of the Ospecific polysaccharide, serological investigation

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