Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting body
The structure was elucidated in this paperNCBI PubMed ID: 25014571Publication DOI: 10.1371/journal.pone.0100380Journal NLM ID: 101285081Publisher: San Francisco, CA: Public Library of Science
Correspondence: Han QB <simonhan
hkbu.edu.hk>; Dai H <daihui
bjmu.edu.cn>
Institutions: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China, Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China, State Key Laboratory of Phytochemistry and Plant Resources in West China, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China, School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China
A polysaccharide named GSP-2 with a molecular size of 32 kDa was isolated from the fruiting bodies of Ganoderma sinense. Its structure was well elucidated, by a combined utilization of chemical and spectroscopic techniques, to be a β-glucan with a backbone of (1→4)- and (1→6)-Glcp, bearing terminal- and (1→3)-Glcp side-chains at O-3 position of (1→6)-Glcp. Immunological assay exhibited that GSP-2 significantly induced the proliferation of BALB/c mice splenocytes with target on only B cells, and enhanced the production of several cytokines in human peripheral blood mononuclear cells and derived dendritic cells. Besides, the fluorescent labeled GSP-2 was phagocytosed by the RAW 264.7 cells and induced the nitric oxide secretion from the cells.
structure elucidation, β-glucan, Ganoderma sinense, fruiting body
Structure type: structural motif or average structure ; 32000
Location inside paper: GSP-2, fig. 2D, Table 3
Trivial name: polysaccharide GSP-2
Compound class: cell wall polysaccharide, glucan
Contained glycoepitopes: IEDB_135614,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_241101,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, GC-MS, UV, methylation analysis, cytokine assay, HMBC, NOESY, HSQC, FT-IR, nitric oxide assay, splenocyte proliferation assay, TFA hydrolysis, HPLC-GPC-ELSD
Biological activity: GSP-2 was found to be B cell dependent immunomodulator without any in vitro stimulating effect to the mouse splenetic T cells, it can significantly stimulate cytokine secretion from PBMCs (Polymixin B) and derive dendritic cells. Fluorescent labeled GSP-2 could be phagocytosis by mouse macrophage like RAW264.7 cells, GSP-2 enhanced the NO production of the cells.
Related record ID(s): 43003, 50029, 50050
NCBI Taxonomy refs (TaxIDs): 36075Reference(s) to other database(s): GTC:G75309HK
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 bDGlcp 103.3 74.0 73.9 78.5 75.1 60.9
6,3,3 bDGlcp 103.3 73.8 75.8 70.2 76.0 60.9
6,3 bDGlcp 103.3 73.8 84.4 70.0 76.3 60.9
6 bDGlcp 103.3 73.9 84.4 68.8 76.0 69.2
bDGlcp 103.3 73.8 76.0 68.8 75.8 69.2
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6 bDGlcp 4.42 3.22 3.36 3.58 3.60 3.61-3.80
6,3,3 bDGlcp 4.72 3.22 3.43 3.44 3.40 3.62-3.82
6,3 bDGlcp 4.70 3.42 3.65 3.35 3.38 3.62-3.82
6 bDGlcp 4.45 3.48 3.65 3.43 3.58 3.77-4.12
bDGlcp 4.41 3.22 3.43 3.44 3.58 3.77-4.12
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6 bDGlcp 103.3/4.42 74.0/3.22 73.9/3.36 78.5/3.58 75.1/3.60 60.9/3.61-3.80
6,3,3 bDGlcp 103.3/4.72 73.8/3.22 75.8/3.43 70.2/3.44 76.0/3.40 60.9/3.62-3.82
6,3 bDGlcp 103.3/4.70 73.8/3.42 84.4/3.65 70.0/3.35 76.3/3.38 60.9/3.62-3.82
6 bDGlcp 103.3/4.45 73.9/3.48 84.4/3.65 68.8/3.43 76.0/3.58 69.2/3.77-4.12
bDGlcp 103.3/4.41 73.8/3.22 76.0/3.43 68.8/3.44 75.8/3.58 69.2/3.77-4.12
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6 | bDGlcp | 4.42 | 3.22 | 3.36 | 3.58 | 3.60 | 3.61
3.80 |
| 6,3,3 | bDGlcp | 4.72 | 3.22 | 3.43 | 3.44 | 3.40 | 3.62
3.82 |
| 6,3 | bDGlcp | 4.70 | 3.42 | 3.65 | 3.35 | 3.38 | 3.62
3.82 |
| 6 | bDGlcp | 4.45 | 3.48 | 3.65 | 3.43 | 3.58 | 3.77
4.12 |
| | bDGlcp | 4.41 | 3.22 | 3.43 | 3.44 | 3.58 | 3.77
4.12 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | bDGlcp | 103.3 | 74.0 | 73.9 | 78.5 | 75.1 | 60.9 |
| 6,3,3 | bDGlcp | 103.3 | 73.8 | 75.8 | 70.2 | 76.0 | 60.9 |
| 6,3 | bDGlcp | 103.3 | 73.8 | 84.4 | 70.0 | 76.3 | 60.9 |
| 6 | bDGlcp | 103.3 | 73.9 | 84.4 | 68.8 | 76.0 | 69.2 |
| | bDGlcp | 103.3 | 73.8 | 76.0 | 68.8 | 75.8 | 69.2 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
(previously named: Ganoderma sinensis)