Taxonomic group: plant, fungi / Streptophyta, Ascomycota
(Phylum: Streptophyta, Ascomycota)
NCBI PubMed ID: 26512632Publication DOI: 10.3390/molecules201019291Journal NLM ID: 100964009Publisher: Basel, Switzerland: MDPI
Correspondence: Qiu ZD <qiuzd
ccucm.edu.cn>; Wang WN <cnweinanwang
163.com>; Yan BX <ybx10528
163.com>; Xu WD <15584452505
163.com>; Qiu Y <ccqy19890810
163.com>; Guo YL <ccyunlong1016
126.com>
Institutions: School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun, China
Compound K (CK), a highly active and bioavailable derivative obtained from protopanaxadiol ginsenosides, displays a wide variety of pharmacological properties, especially antitumor activity. However, the inadequacy of natural sources limits its application in the pharmaceutical industry. In this study, we firstly discovered that Cordyceps sinensis was a potent biocatalyst for the biotransformation of ginsenoside Rb1 into CK. After a series of investigations on the biotransformation parameters, an optimal composition of the biotransformation culture was found to be lactose, soybean powder and MgSO4 without controlling the pH. Also, an optimum temperature of 30 °C for the biotransformation process was suggested in a range of 25 °C-50 °C. Then, a biotransformation pathway of Rb1→Rd→F2→CK was established using high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Our results demonstrated that the molar bioconversion rate of Rb1 to CK was more than 82% and the purity of CK produced by C. sinensis under the optimized conditions was more than 91%. In conclusion, the combination of C. sinensis and the optimized conditions is applicable for the industrial preparation of CK for medicinal purposes
optimization, Cordyceps sinensis, biotransformation, compound K, ginsenoside Rb1
Structure type: oligomer
Location inside paper: Table 1, F2
Trivial name: ginsenoside-F2, ginsenoside F2
Compound class: saponin glycoside, glycoside, triterpene glycoside, triterpenoid glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, TLC, extraction, ESI-QTOF-MS, cell growth, HPLC-MS, evaporation, sonication
Synthetic data: enzymatic in vivo
Related record ID(s): 44247, 47977, 47978, 47979, 47981, 47982, 47983, 47984, 47985, 47986, 47987
NCBI Taxonomy refs (TaxIDs): 4054,
72228Reference(s) to other database(s): CCSD:
27076, CBank-STR:2956
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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